The purpose of this study was to compare the co-administration of the probiotic Bacillus coagulans GBI-30, 6086 (BC30) with β-hydroxy-β-methylbutyrate (HMB) calcium (CaHMB) to CaHMB alone on the inflammatory response and muscle integrity during 40-days of intense military training. Soldiers were randomly assigned to one of two groups: CaHMB with BC30 (CaHMBBC30; n=9) or CaHMB with placebo (CaHMBPL, n=9). A third group of participants served as a control (CTL; n=8). During the first 28 days soldiers were garrisoned on base and participated in the same training tasks. During the final 2 weeks soldiers navigated 25-30 km per night in difficult terrain carrying ~35 kg of equipment. All assessments (blood draws and diffusion tensor imaging to assess muscle integrity) were conducted prior to and approximately 12 hours following final supplement consumption. Analysis of covariance was used to analyze all blood and muscle measures. Significant attenuations were noted in IL-1β, IL-2, IL-6, CX3CL1, and TNF-α for both CaHMBBC30 and CaHMBPL compared to CTL. Plasma IL-10 concentrations were significantly attenuated for CaHMBBC30 compared to CTL only. A significant decrease in apparent diffusion coefficients was also observed for CaHMBBC30 compared to CaHMBPL. Results provide further evidence that HMB supplementation may attenuate the inflammatory response to intense training, and that the combination of the probiotic BC30 with CaHMB may be more beneficial than CaHMB alone in maintaining muscle integrity during intense military training.
- Diffusion Tensor Imaging
- Copyright © 2016, Journal of Applied Physiology