Untrained Young Men Have Dysfunctional HDL Compared to Strength Trained Men Irrespective of Overweight/Obesity Status

Christian K Roberts, Michael Katiraie, Daniel M Croymans, Otto O. Yang, Theodoros Kelesidis


We examined the impact of strength fitness and body weight on the redox properties of high-density lipoprotein (HDL) and associations with indices of vascular and metabolic health. 90 young males were categorized into 3 groups: overweight untrained (OU) (n=30, BMI 30.7±2.1 kg/m2), overweight trained (OT) (n=30, BMI 29.0±1.9, ≥4 d/wk resistance training (RT)) and lean trained (LT) (n=30, BMI 23.7±1.4, ≥4 d/wk RT). Using a novel assay based on HDL mediated rate of oxidation of dihydrorhodamine (DOR), we determined the functional (redox) properties of HDL and correlations between DOR and indices of vascular and metabolic health were examined in the cohort. DOR was significantly lower in both trained groups compared with the untrained group (LT 1.04±0.49, OT 1.39±0.57, OU 1.80±0.74, LT vs. OU: P<0.00001; OT vs. OU, P=0.02), however OT was not significantly different from LT. DOR was negatively associated with HDL-C (R=-0.64), relative strength (R=-0.42), sex hormone-binding globulin (SHBG) (R=-0.42), and testosterone (R=-0.35) (all P≤0.001), while DOR was positively associated with triglycerides (R=0.39, P=0.002), oxLDL (R=0.32), body mass index (R=0.43), total mass (R=0.35), total fat mass (R=0.42), waist circumference (R=0.45) and trunk fat mass (R=0.42) (all P≤0.001). Chronic RT is associated with improved HDL redox activity. This may contribute to RT's beneficial effects on reducing cardiovascular disease risk, irrespective of body weight status.

  • dysfunctional HDL
  • dihydrorhodamine
  • resistance training
  • exercise
  • obesity