The carbohydrate metabolism of human spermatozoa in seminal plasma was studied by measuring the fructose utilization and lactate production of semen specimens. The recovery of two moles of lactate for each mole of fructose utilized suggests that, as in Ringer's solution, glycolysis is the main pathway of carbohydrate metabolism in this system. Sperm concentration and initial fructose level were found to influence the fructolytic activity of this system but there was no relationship apparent between sperm motility and fructolysis. Significant differences in fructose utilization were demonstrated among the donors in this study. This was further examined by a seminal plasma reversal experiment which clearly indicated that the plasma has a marked influence on fructolysis and that the plasmas of individual donors have strikingly different effects on sperm metabolism.
Submitted on May 9, 1958
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