HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free All Versions of this Article: 99/4/1359    most recent 00197.2005v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (19) Citing Articles via Google Scholar Google Scholar Articles by Cluberton, L. J. Articles by Hargreaves, M. Search for Related Content PubMed PubMed Citation Articles by Cluberton, L. J. Articles by Hargreaves, M.

Effect of carbohydrate ingestion on exercise-induced alterations in metabolic gene expression

Centre for Physical Activity and Nutrition, School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria, Australia

Submitted 17 February 2005 ; accepted in final form 30 May 2005

Skeletal muscle possesses a high degree of plasticity and can adapt to both the physical and metabolic challenges that it faces. An acute bout of exercise is sufficient to induce the expression of a variety of metabolic genes, such as GLUT4, pyruvate dehydrogenase kinase 4 (PDK-4), uncoupling protein-3 (UCP3), and peroxisome proliferator-activated receptor- coactivator 1 (PGC-1). Reducing muscle glycogen levels before exercise potentiates the effect of exercise on many genes. Similarly, altered substrate availability induces transcription of many of these genes. The purpose of this study was to determine whether glucose ingestion attenuates the exercise-induced increase in a variety of exercise-responsive genes. Six male subjects (28 ± 7 yr; 83 ± 3 kg; peak pulmonary oxygen uptake = 46 ± 6 ml·kg^–1·min^–1) performed 60 min of cycling at 74 ± 2% of peak pulmonary oxygen uptake on two separate occasions. On one occasion, subjects ingested a 6% carbohydrate drink. On the other occasion, subjects ingested an equal volume of a sweet placebo. Muscle samples were obtained from vastus lateralis at rest, immediately after exercise, and 3 h after exercise. PDK-4, UCP3, PGC-1, and GLUT4 mRNA levels were measured on these samples using real-time RT-PCR. Glucose ingestion attenuated (P < 0.05) the exercise-induced increase in PDK-4 and UCP3 mRNA. A similar trend (P = 0.09) was observed for GLUT4 mRNA. In contrast, PGC-1 mRNA increased following exercise to the same extent in both conditions. These data suggest that glucose availability can modulate the effect of exercise on metabolic gene expression.

transcription; skeletal muscle

This article has been cited by other articles:

				F. W. Booth and J. M. Company Local adaptation in human trained skeletal muscle could preferentially bind blood interleukin-6 Exp Physiol, November 1, 2009; 94(11): 1099 - 1100. [Full Text] [PDF]

				T. C. A. Akerstrom, C. P. Fischer, P. Plomgaard, C. Thomsen, G. van Hall, and B. K. Pedersen Glucose ingestion during endurance training does not alter adaptation J Appl Physiol, June 1, 2009; 106(6): 1771 - 1779. [Abstract] [Full Text] [PDF]

				J. P. Morton, L. Croft, J. D. Bartlett, D. P. M. MacLaren, T. Reilly, L. Evans, A. McArdle, and B. Drust Reduced carbohydrate availability does not modulate training-induced heat shock protein adaptations but does upregulate oxidative enzyme activity in human skeletal muscle J Appl Physiol, May 1, 2009; 106(5): 1513 - 1521. [Abstract] [Full Text] [PDF]

				A. S. Mathai, A. Bonen, C. R. Benton, D. L. Robinson, and T. E. Graham Rapid exercise-induced changes in PGC-1{alpha} mRNA and protein in human skeletal muscle J Appl Physiol, October 1, 2008; 105(4): 1098 - 1105. [Abstract] [Full Text] [PDF]

				E. Karnieli and M. Armoni Transcriptional regulation of the insulin-responsive glucose transporter GLUT4 gene: from physiology to pathology Am J Physiol Endocrinol Metab, July 1, 2008; 295(1): E38 - E45. [Abstract] [Full Text] [PDF]

				Z. Arany, B. K. Wagner, Y. Ma, J. Chinsomboon, D. Laznik, and B. M. Spiegelman Gene expression-based screening identifies microtubule inhibitors as inducers of PGC-1{alpha} and oxidative phosphorylation PNAS, March 25, 2008; 105(12): 4721 - 4726. [Abstract] [Full Text] [PDF]

				H. J. Green, T. A. Duhamel, G. P. Holloway, J. W. Moule, D. W. Ranney, A. R. Tupling, and J. Ouyang Rapid upregulation of GLUT-4 and MCT-4 expression during 16 h of heavy intermittent cycle exercise Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2008; 294(2): R594 - R600. [Abstract] [Full Text] [PDF]

				Z. Arany, M. Novikov, S. Chin, Y. Ma, A. Rosenzweig, and B. M. Spiegelman Transverse aortic constriction leads to accelerated heart failure in mice lacking PPAR-{gamma} coactivator 1{alpha} PNAS, June 27, 2006; 103(26): 10086 - 10091. [Abstract] [Full Text] [PDF]