HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free All Versions of this Article: 95/3/1201    most recent 00257.2003v2 00257.2003v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (20) Citing Articles via Google Scholar Google Scholar Articles by Nordsborg, N. Articles by Pilegaard, H. Search for Related Content PubMed PubMed Citation Articles by Nordsborg, N. Articles by Pilegaard, H.

Effect of high-intensity training on exercise-induced gene expression specific to ion homeostasis and metabolism

¹Institute of Exercise and Sport Sciences and ²Laboratory of Cellular and Molecular Biology, Copenhagen Muscle Research Centre, August Krogh Institute, University of Copenhagen, 2100 Copenhagen, Denmark

Submitted 12 March 2003 ; accepted in final form 15 May 2003

Changes in gene expression during recovery from high-intensity, intermittent, one-legged exercise were studied before and after 5.5 wk of training. Genes related to metabolism, as well as Na⁺, K⁺, and pH homeostasis, were selected for analyses. After the same work was performed before and after the training period, several muscle biopsies were obtained from vastus lateralis muscle. In the untrained state, the Na⁺-K⁺-ATPase ₁-subunit mRNA level was approximately threefold higher (P < 0.01) at 0, 1, and 3 h after exercise, relative to the preexercise resting level. After 3-5 h of recovery in the untrained state, pyruvate dehydrogenase kinase 4 and hexokinase II mRNA levels were elevated 13-fold (P < 0.001) and 6-fold (P < 0.01), respectively. However, after the training period, only pyruvate dehydrogenase kinase 4 mRNA levels were elevated (P < 0.05) during the recovery period. No changes in resting mRNA levels were observed as a result of training. In conclusion, cellular adaptations to high-intensity exercise training may, in part, be induced by transcriptional regulation. After training, the transcriptional response to an exercise bout at a given workload is diminished.

sodium-potassium-adenosine 5'-triphosphatase; lactate/hydrogen ion transport; messenger ribonucleic acid; regulation of substrate utilization; glycolysis

This article has been cited by other articles:

				K. T. Murphy, I. Medved, M. J. Brown, D. Cameron-Smith, and M. J. McKenna Antioxidant treatment with N-acetylcysteine regulates mammalian skeletal muscle Na+-K+-ATPase {alpha} gene expression during repeated contractions Exp Physiol, December 1, 2008; 93(12): 1239 - 1248. [Abstract] [Full Text] [PDF]

				K. De Bock, W. Derave, B. O. Eijnde, M. K. Hesselink, E. Koninckx, A. J. Rose, P. Schrauwen, A. Bonen, E. A. Richter, and P. Hespel Effect of training in the fasted state on metabolic responses during exercise with carbohydrate intake J Appl Physiol, April 1, 2008; 104(4): 1045 - 1055. [Abstract] [Full Text] [PDF]

				R. J. Aughey, K. T. Murphy, S. A. Clark, A. P. Garnham, R. J. Snow, D. Cameron-Smith, J. A. Hawley, and M. J. McKenna Muscle Na+-K+-ATPase activity and isoform adaptations to intense interval exercise and training in well-trained athletes J Appl Physiol, July 1, 2007; 103(1): 39 - 47. [Abstract] [Full Text] [PDF]

				B. Phakdeekitcharoen, S. Phudhichareonrat, C. Pookarnjanamorakot, C. Kijkunasathian, N. Tubtong, W. Kittikanokrat, and P. Radinahamed Thyroid Hormone Increases mRNA and Protein Expression of Na+-K+-ATPase {alpha}2 and {beta}1 Subunits in Human Skeletal Muscles J. Clin. Endocrinol. Metab., January 1, 2007; 92(1): 353 - 358. [Abstract] [Full Text] [PDF]

				K. T. Murphy, A. C. Petersen, C. Goodman, X. Gong, J. A. Leppik, A. P. Garnham, D. Cameron-Smith, R. J. Snow, and M. J. McKenna Prolonged submaximal exercise induces isoform-specific Na+-K+-ATPase mRNA and protein responses in human skeletal muscle Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2006; 290(2): R414 - R424. [Abstract] [Full Text] [PDF]

				J. Shearer, T. E. Graham, D. S. Battram, D. L. Robinson, E. A. Richter, R. J. Wilson, and M. Bakovic Glycogenin activity and mRNA expression in response to volitional exhaustion in human skeletal muscle J Appl Physiol, September 1, 2005; 99(3): 957 - 962. [Abstract] [Full Text] [PDF]

				N. Nordsborg, M. Thomassen, C. Lundby, H. Pilegaard, and J. Bangsbo Contraction-induced increases in Na+-K+-ATPase mRNA levels in human skeletal muscle are not amplified by activation of additional muscle mass Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2005; 289(1): R84 - R91. [Abstract] [Full Text] [PDF]

				Y. Yang, A. Creer, B. Jemiolo, and S. Trappe Time course of myogenic and metabolic gene expression in response to acute exercise in human skeletal muscle J Appl Physiol, May 1, 2005; 98(5): 1745 - 1752. [Abstract] [Full Text] [PDF]

				C. P. Fischer, P. Plomgaard, A. K. Hansen, H. Pilegaard, B. Saltin, and B. K. Pedersen Endurance training reduces the contraction-induced interleukin-6 mRNA expression in human skeletal muscle Am J Physiol Endocrinol Metab, December 1, 2004; 287(6): E1189 - E1194. [Abstract] [Full Text] [PDF]

				P. J. LeBlanc, S. J. Peters, R. J. Tunstall, D. Cameron-Smith, and G. J. F. Heigenhauser Effects of aerobic training on pyruvate dehydrogenase and pyruvate dehydrogenase kinase in human skeletal muscle J. Physiol., June 1, 2004; 557(2): 559 - 570. [Abstract] [Full Text] [PDF]

				K. T. Murphy, R. J. Snow, A. C. Petersen, R. M. Murphy, J. Mollica, J. S. Lee, A. P. Garnham, R. J. Aughey, J. A. Leppik, I. Medved, et al. Intense exercise up-regulates Na+,K+-ATPase isoform mRNA, but not protein expression in human skeletal muscle J. Physiol., April 15, 2004; 556(2): 507 - 519. [Abstract] [Full Text] [PDF]