Journal of Applied Physiology Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 93: 1411-1418, 2002. First published June 30, 2002; doi:10.1152/japplphysiol.00251.2002
8750-7587/02 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
93/4/1411    most recent
00251.2002v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (31)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Steiner, D. R. S.
Right arrow Articles by Wood, J. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Steiner, D. R. S.
Right arrow Articles by Wood, J. G.
Vol. 93, Issue 4, 1411-1418, October 2002

Interaction between reactive oxygen species and nitric oxide in the microvascular response to systemic hypoxia

Dawn R. S. Steiner, Norberto C. Gonzalez, and John G. Wood

Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, Kansas 66160

Systemic hypoxia results in oxidative stress due to a change in the reactive oxygen species (ROS)-nitric oxide (NO) balance. These experiments explored two mechanisms for the altered ROS-NO balance: 1) decreased NO synthesis by NO synthase due to limited O2 substrate availability and 2) increased superoxide generation. ROS levels and leukocyte adherence in mesenteric venules of rats during hypoxia were studied in the absence and presence of an NO donor [spermine NONOate (SNO)] and of the NO precursor L-arginine. We hypothesized that if the lower NO levels during hypoxia were due to O2 substrate limitation, L-arginine would not prevent hypoxia-induced microvascular responses. Graded hypoxia (produced by breathing 15, 10, and 7.5% O2) increased both ROS (123 ± 6, 148 ± 11, and 167 ± 3% of control) and leukocyte adherence. ROS levels during breathing of 10 and 7.5% O2 were significantly attenuated by SNO (105 ± 6 and 108 ± 3%, respectively) and L-arginine (117 ± 5 and 115 ± 2%, respectively). Both interventions reduced leukocyte adherence by similar degrees. The fact that the effects of L-arginine were similar to those of SNO does not support the idea that NO generation is impaired in hypoxia and suggests that tissue NO levels are depleted by the increased ROS during hypoxia.

mesenteric venules; leukocyte adherence; superoxide; dihydrorhodamine


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. G. Julian, H. L. Galan, M. J. Wilson, W. DeSilva, D. Cioffi-Ragan, J. Schwartz, and L. G. Moore
Lower uterine artery blood flow and higher endothelin relative to nitric oxide metabolite levels are associated with reductions in birth weight at high altitude
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2008; 295(3): R906 - R915.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Bertuglia
Intermittent hypoxia modulates nitric oxide-dependent vasodilation and capillary perfusion during ischemia-reperfusion-induced damage
Am J Physiol Heart Circ Physiol, April 1, 2008; 294(4): H1914 - H1922.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
N. C. Gonzalez, J. Allen, V. G. Blanco, E. J. Schmidt, N. van Rooijen, and J. G. Wood
Alveolar macrophages are necessary for the systemic inflammation of acute alveolar hypoxia
J Appl Physiol, October 1, 2007; 103(4): 1386 - 1394.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. L. Clanton
Hypoxia-induced reactive oxygen species formation in skeletal muscle
J Appl Physiol, June 1, 2007; 102(6): 2379 - 2388.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
N. C. Gonzalez, J. Allen, E. J. Schmidt, A. J. Casillan, T. Orth, and J. G. Wood
Role of the renin-angiotensin system in the systemic microvascular inflammation of alveolar hypoxia
Am J Physiol Heart Circ Physiol, May 1, 2007; 292(5): H2285 - H2294.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. A. Phillips, E. B. Olson, J. H. Lombard, and B. J. Morgan
Chronic intermittent hypoxia alters NE reactivity and mechanics of skeletal muscle resistance arteries
J Appl Physiol, April 1, 2006; 100(4): 1117 - 1123.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
G. E. Foster, D. C. McKenzie, W. K. Milsom, and A. W. Sheel
Effects of two protocols of intermittent hypoxia on human ventilatory, cardiovascular and cerebral responses to hypoxia
J. Physiol., September 1, 2005; 567(2): 689 - 699.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. Orth, J. A. Allen, J. G. Wood, and N. C. Gonzalez
Plasma from conscious hypoxic rats stimulates leukocyte-endothelial interactions in normoxic cremaster venules
J Appl Physiol, July 1, 2005; 99(1): 290 - 297.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
T. A. Orth, J. A. Allen, J. G. Wood, and N. C. Gonzalez
Exercise training prevents the inflammatory response to hypoxia in cremaster venules
J Appl Physiol, June 1, 2005; 98(6): 2113 - 2118.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
B. Friesenecker, A. G. Tsai, M. W. Dunser, A. J. Mayr, J. Martini, H. Knotzer, W. Hasibeder, and M. Intaglietta
Oxygen distribution in microcirculation after arginine vasopressin-induced arteriolar vasoconstriction
Am J Physiol Heart Circ Physiol, October 1, 2004; 287(4): H1792 - H1800.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. A. Phillips, I. Drenjancevic-Peric, J. C. Frisbee, and J. H. Lombard
Chronic AT1 receptor blockade alters mechanisms mediating responses to hypoxia in rat skeletal muscle resistance arteries
Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H545 - H552.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. A. Phillips, E. B. Olson, B. J. Morgan, and J. H. Lombard
Chronic intermittent hypoxia impairs endothelium-dependent dilation in rat cerebral and skeletal muscle resistance arteries
Am J Physiol Heart Circ Physiol, January 1, 2004; 286(1): H388 - H393.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. Dix, T. Orth, J. Allen, J. G. Wood, and N. C. Gonzalez
Activation of mast cells by systemic hypoxia, but not by local hypoxia, mediates increased leukocyte-endothelial adherence in cremaster venules
J Appl Physiol, December 1, 2003; 95(6): 2495 - 2502.
[Abstract] [Full Text]

Home page
 Am. J. Pathol.Home page
N. N. C. Tam, Y. Gao, Y.-K. Leung, and S.-M. Ho
Androgenic Regulation of Oxidative Stress in the Rat Prostate: Involvement of NAD(P)H Oxidases and Antioxidant Defense Machinery during Prostatic Involution and Regrowth
Am. J. Pathol., December 1, 2003; 163(6): 2513 - 2522.
[Abstract] [Full Text]

Home page
 J. Appl. Physiol.Home page
A. J. Casillan, N. C. Gonzalez, J. S. Johnson, D. R. S. Steiner, and J. G. Wood
Mesenteric microvascular inflammatory responses to systemic hypoxia are mediated by PAF and LTB4
J Appl Physiol, June 1, 2003; 94(6): 2313 - 2322.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
S. Shah, J. Allen, J. G. Wood, and N. C. Gonzalez
Dissociation between skeletal muscle microvascular PO2 and hypoxia-induced microvascular inflammation
J Appl Physiol, June 1, 2003; 94(6): 2323 - 2329.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. R. S. Steiner, N. C. Gonzalez, and J. G. Wood
Mast cells mediate the microvascular inflammatory response to systemic hypoxia
J Appl Physiol, January 1, 2003; 94(1): 325 - 334.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Bertuglia and A. Giusti
Role of nitric oxide in capillary perfusion and oxygen delivery regulation during systemic hypoxia
Am J Physiol Heart Circ Physiol, February 1, 2005; 288(2): H525 - H531.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online