Journal of Applied Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 92: 2429-2438, 2002. First published February 8, 2002; doi:10.1152/japplphysiol.01024.2001
8750-7587/02 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
92/6/2429    most recent
01024.2001v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (15)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Burelle, Y.
Right arrow Articles by Hochachka, P. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burelle, Y.
Right arrow Articles by Hochachka, P. W.
Vol. 92, Issue 6, 2429-2438, June 2002

Endurance training induces muscle-specific changes in mitochondrial function in skinned muscle fibers

Yan Burelle and Peter W. Hochachka

Departments of Zoology and Radiology, and Sports Medicine Division, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4

The present study was conducted to investigate the potential role of changes in the apparent Km for ADP and in the functional coupling of the creatine (Cr) kinase (CK) system (CK efficiency) in explaining the tighter integration of ATP supply and demand after exercise training. Mitochondrial function was assessed in saponin-skinned fibers from the soleus and the deep red portion of the medial gastrocnemius isolated from trained (T; treadmill running, 5 days/wk, 4 wk) and control (C) female Sprague-Dawley rats. In the soleus, Vmax in the presence of 1 mM ADP was increased by 21% after training (5.9 ± 0.2 vs. 4.7 ± 0.4 nmol O2 · min-1 · mg dry wt-1, P < 0.05). This was accompanied by no change in the Km for ADP measured in the absence of Cr (146 ± 9 vs. 149 ± 13 µM in T and C, respectively) and in its presence (50 ± 4 vs. 48 ± 6 µM in T and C, respectively) and in CK efficiency [Km (+Cr)/Km (-Cr)]. In contrast, in the red gastrocnemius, training decreased, by 35%, the apparent Km for ADP in the absence (83 ± 5 vs. 129 ± 9 µM, P < 0.01) of Cr, without affecting Vmax (6.2 ± 0.4 vs. 6.7 ± 0.3 nmol O2 · min-1 · mg dry wt-1 in T and C, respectively) and CK efficiency. These results thus suggest that training induces muscle-specific adaptations of mitochondrial function and that a change in the intrinsic sensitivity of mitochondria to ADP could at least partly explain the tighter integration of ATP and demand commonly observed after training.

exercise training; oxidative phosphorylation; creatine kinase system


This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Picard, K. Csukly, M.-E. Robillard, R. Godin, A. Ascah, C. Bourcier-Lucas, and Y. Burelle
Resistance to Ca2+-induced opening of the permeability transition pore differs in mitochondria from glycolytic and oxidative muscles
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2008; 295(2): R659 - R668.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
K. Csukly, A. Ascah, J. Matas, P. F. Gardiner, E. Fontaine, and Y. Burelle
Muscle denervation promotes opening of the permeability transition pore and increases the expression of cyclophilin D
J. Physiol., July 1, 2006; 574(1): 319 - 327.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
B. Walsh, R. B. Hooks, J. E. Hornyak, L. G. Koch, S. L. Britton, and M. C. Hogan
Enhanced mitochondrial sensitivity to creatine in rats bred for high aerobic capacity
J Appl Physiol, June 1, 2006; 100(6): 1765 - 1769.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. A. Kindig, B. Walsh, R. A. Howlett, C. M. Stary, and M. C. Hogan
Relationship between intracellular PO2 recovery kinetics and fatigability in isolated single frog myocytes
J Appl Physiol, June 1, 2005; 98(6): 2316 - 2319.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
N. Gueguen, L. Lefaucheur, P. Ecolan, M. Fillaut, and P. Herpin
Ca2+-activated myosin-ATPases, creatine and adenylate kinases regulate mitochondrial function according to myofibre type in rabbit
J. Physiol., May 1, 2005; 564(3): 723 - 735.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
P. McDonough, B. J. Behnke, T. I. Musch, and D. C. Poole
Effects of chronic heart failure in rats on the recovery of microvascular PO2 after contractions in muscles of opposing fibre type
Exp Physiol, July 1, 2004; 89(4): 473 - 485.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
T. Andrienko, A. V. Kuznetsov, T. Kaambre, Y. Usson, A. Orosco, F. Appaix, T. Tiivel, P. Sikk, M. Vendelin, R. Margreiter, et al.
Metabolic consequences of functional complexes of mitochondria, myofibrils and sarcoplasmic reticulum in muscle cells
J. Exp. Biol., June 15, 2003; 206(12): 2059 - 2072.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
V. Saks, A. Kuznetsov, T. Andrienko, Y. Usson, F. Appaix, K. Guerrero, T. Kaambre, P. Sikk, M. Lemba, and M. Vendelin
Heterogeneity of ADP Diffusion and Regulation of Respiration in Cardiac Cells
Biophys. J., May 1, 2003; 84(5): 3436 - 3456.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online