Journal of Applied Physiology http://www.adinstruments.com/labchart/faseb
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 92: 2396-2402, 2002. First published February 8, 2002; doi:10.1152/japplphysiol.00755.2001
8750-7587/02 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
92/6/2396    most recent
00755.2001v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Frohock, J. I.
Right arrow Articles by Salathe, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Frohock, J. I.
Right arrow Articles by Salathe, M.
Vol. 92, Issue 6, 2396-2402, June 2002

Effects of albuterol enantiomers on ciliary beat frequency in ovine tracheal epithelial cells

Jeffrey I. Frohock, Corrine Wijkstrom-Frei, and Matthias Salathe

Division of Pulmonary and Critical Care Medicine, University of Miami School of Medicine, Miami, Florida 33136

beta 2-Adrenergic agonists stimulate ciliary beat frequency (CBF), an integral part of mucociliary clearance. To evaluate the differential effects of albuterol enantiomers and their racemic mixture on ciliary function, CBF and intracellular calcium were measured at room temperature from single ovine airway epithelial cells with use of digital videomicroscopy. Baseline CBF was 7.2 ± 0.2 (SE) Hz (n = 80 measurements). R-albuterol (10 µM to 1 mM) stimulated CBF in a dose-dependent manner to maximally 24.4 ± 5.4% above baseline. Racemic albuterol stimulated CBF to maximally 12.8 ± 3.6% above baseline, a significantly lower increase compared with R-albuterol alone, despite identical R-enantiomer amounts in both groups. Simultaneous recordings of intracellular calcium concentration and CBF from single cells indicated that the CBF increase in response to R-albuterol was mediated through beta -receptors and stimulation of protein kinase A, in a calcium-dependent and -independent fashion. S-albuterol had a negligible effect on CBF and did not change intracellular calcium. Together, these results suggest that R-albuterol is more efficacious than racemic albuterol in stimulating CBF. Thus S-albuterol may interfere with the ability of R-albuterol to increase CBF.

airway; calcium; beta-agonists; signaling


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
A. Schmid, G. Bai, N. Schmid, M. Zaccolo, L. E. Ostrowski, G. E. Conner, N. Fregien, and M. Salathe
Real-time analysis of cAMP-mediated regulation of ciliary motility in single primary human airway epithelial cells
J. Cell Sci., October 15, 2006; 119(20): 4176 - 4186.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
P. Delmotte and M. J. Sanderson
Ciliary Beat Frequency Is Maintained at a Maximal Rate in the Small Airways of Mouse Lung Slices
Am. J. Respir. Cell Mol. Biol., July 1, 2006; 35(1): 110 - 117.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
T. G. O'Riordan, W. Mao, L. B. Palmer, and J. J. Chen
Assessing the Effects of Racemic and Single-Enantiomer Albuterol on Airway Secretions in Long-term Intubated Patients
Chest, January 1, 2006; 129(1): 124 - 132.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
B. N. Chorley, Y. Li, S. Fang, J.-A. Park, and K. B. Adler
(R)-Albuterol Elicits Antiinflammatory Effects in Human Airway Epithelial Cells via iNOS
Am. J. Respir. Cell Mol. Biol., January 1, 2006; 34(1): 119 - 127.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
J. R. Sabater, T. A. Lee, and W. M. Abraham
Comparative Effects of Salmeterol, Albuterol, and Ipratropium on Normal and Impaired Mucociliary Function in Sheep
Chest, November 1, 2005; 128(5): 3743 - 3749.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online