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J Appl Physiol 92: 2045-2052, 2002. First published December 21, 2001; doi:10.1152/japplphysiol.00673.2001
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Vol. 92, Issue 5, 2045-2052, May 2002

Early changes of type 2B fibers after denervation of rat EDL skeletal muscle

Elena Germinario1, Alessandra Esposito1, Aram Megighian1, Menotti Midrio1, Donatella Biral2, Romeo Betto2, and Daniela Danieli-Betto1

1 Dipartimento di Anatomia e Fisiologia Umana, and 2 Consiglio Nazionale delle Ricerche, Centro di Studio per la Biologia e la Fisiopatologia Muscolare, I-35131 Padova, Italy

Skeletal muscle type 2B fibers normally receive a moderate level of motoneuron discharge. As a consequence, we hypothesize that type 2B fiber properties should be less sensitive to the absence of the nerve. Therefore, we have investigated the response of sarcoplasmic reticulum and myofibrillar proteins of type 2B fibers isolated from rat extensor digitorum longus muscle after denervation (2 and 7 days). Single fibers were identified by SDS-PAGE of myosin heavy chain isoforms. Electrophysiological and isometric contractile properties of the whole muscle were also analyzed. The pCa-tension relationship of type 2B single fibers was shifted to the left at 2 days and to right at 7 days after denervation, with significant differences in the Hill coefficients and pCa threshold values in 2- vs. 7-day-denervated fibers. The sarcoplasmic reticulum Ca2+ uptake capacity and rate significantly decreased after 2 days of denervation, whereas both increased at 7 days. Caffeine sensitivity of sarcoplasmic reticulum Ca2+ release was transitory and markedly increased in 2-day-denervated fibers. Our results indicate that type 2B fiber functional properties are highly sensitive to the interruption of nerve supply. Moreover, most of 2-day-denervated changes were reverted at 7 days.

calcium sensitivity; chemically skinned muscle fibers; sarcoplasmic reticulum; extensor digitorum longus


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