Journal of Applied Physiology Journal of Neurophysiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 92: 1443-1450, 2002. First published December 14, 2001; doi:10.1152/japplphysiol.00782.2001
8750-7587/02 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
92/4/1443    most recent
00782.2001v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stamenovic, D.
Right arrow Articles by Wang, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stamenovic, D.
Right arrow Articles by Wang, N.
Vol. 92, Issue 4, 1443-1450, April 2002

Effect of the cytoskeletal prestress on the mechanical impedance of cultured airway smooth muscle cells

Dimitrije Stamenovic'1, Zhuangli Liang1, Jianxin Chen2, and Ning Wang2

1 Department of Biomedical Engineering, Boston University, Boston 02215; and 2 Physiology Program, Department of Environmental Health, Harvard School of Public Health, Boston, Massachusetts 02115

We investigated the effect of the cytoskeletal prestress (P) on the elastic and frictional properties of cultured human airway smooth muscle cells during oscillatory loading; P is preexisting tensile stress in the actin cytoskeleton generated by the cell contractile apparatus. We oscillated (0.1 Hz, 6 Pa peak to peak) small ferromagnetic beads bound to integrin receptors and computed the storage (elastic) modulus (G') and the loss (frictional) modulus (G") from the applied torque and the corresponding bead rotation. All measurements were done at baseline and after cells were treated with graded doses of either histamine (0.1, 1, 10 µM) or isoproterenol (0.01, 0.1, 1, 10 µM). Values for P for these concentrations were taken from a previous study (Wang et al., Am J Physiol Cell Physiol, in press). It was found that G' and G", as well as P, increased/decreased with increasing doses of histamine/isoproterenol. Both G' and G" exhibited linear dependences on P: G'(Pa) = 0.20P + 82 and G"(Pa) = 0.05P + 32. The dependence of G' on P is consistent with our previous findings and with the behavior of stress-supported structures. The dependence of G" on P is a novel finding. It could be attributed to a variety of mechanisms. Some of those mechanisms are discussed in detail. We concluded that, in addition to the central mechanisms by which stress-supported structures develop mechanical stresses, other mechanisms might need to be invoked to fully explain the observed dependence of the cell mechanical properties on the state of cell contractility.

storage modulus; loss modulus; oscillatory cytometry; actin network; cytoskeletal mechanics


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
M. A. Griffin, H. Feng, M. Tewari, P. Acosta, M. Kawana, H. L. Sweeney, and D. E. Discher
{gamma}-Sarcoglycan deficiency increases cell contractility, apoptosis and MAPK pathway activation but does not affect adhesion
J. Cell Sci., April 1, 2005; 118(7): 1405 - 1416.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. A. Griffin, S. Sen, H. L. Sweeney, and D. E. Discher
Adhesion-contractile balance in myocyte differentiation
J. Cell Sci., November 15, 2004; 117(24): 5855 - 5863.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
X. Trepat, M. Grabulosa, F. Puig, G. N. Maksym, D. Navajas, and R. Farre
Viscoelasticity of human alveolar epithelial cells subjected to stretch
Am J Physiol Lung Cell Mol Physiol, November 1, 2004; 287(5): L1025 - L1034.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. E. Ingber
Tensegrity I. Cell structure and hierarchical systems biology
J. Cell Sci., April 1, 2003; 116(7): 1157 - 1173.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online