| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 School of Human Movement, Recreation and Performance, and 2 School of Life Sciences and Technology, Centre for Rehabilitation, Exercise and Sports Science, Victoria University of Technology, Melbourne, 8001 Victoria, Australia
Little is
known about fatigue and training effects on sarcoplasmic reticulum (SR)
function in human muscle, and we therefore investigated this in eight
untrained controls (UT), eight endurance-trained (ET), and eight
resistance-trained athletes (RT). Muscle biopsies (vastus lateralis)
taken at rest and after 50 maximal quadriceps contractions (180°/s,
0.5 Hz) were analyzed for fiber composition, metabolites and maximal SR
Ca2+ release, Ca2+ uptake, and
Ca2+-ATPase activity. Fatigue reduced (P < 0.05) Ca2+ release (42.1 ± 3.8%, 43.4 ± 3.9%,
31.3 ± 6.1%), Ca2+ uptake (43.0 ± 5.2%,
34.1 ± 4.6%, 28.4 ± 2.8%), and Ca2+-ATPase
activity (38.6 ± 4.2%, 48.5 ± 5.7%, 29.6 ± 5.0%),
in UT, RT, and ET, respectively. These decreases were correlated with fatigability and with type II fiber proportion (P < 0.05). Resting SR measures were correlated with type II proportion
(r 
0.51, P < 0.05). ET had lower resting
Ca2+ release, Ca2+ uptake, and
Ca2+-ATPase (P < 0.05) than UT and RT
(P < 0.05), probably because of their lower type II
proportion; only minor effects were found in RT. Thus SR function is
markedly depressed with fatigue in controls and in athletes, is
dependent on fiber type, and appears to be minimally affected by
chronic training status.
calcium release; calcium uptake; calcium-ATPase; fiber type; exercise
This article has been cited by other articles:
|
|
 |
|
  S. Matsunaga, T. Mishima, T. Yamada, S. Inashima, and M. Wada Alterations in in vitro function and protein oxidation of rat sarcoplasmic reticulum Ca2+-ATPase during recovery from high-intensity exercise Exp Physiol, March 1, 2008; 93(3): 426 - 433. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Allen, G. D. Lamb, and H. Westerblad Skeletal Muscle Fatigue: Cellular Mechanisms Physiol Rev, January 1, 2008; 88(1): 287 - 332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Allen, G. D. Lamb, and H. Westerblad Impaired calcium release during fatigue J Appl Physiol, January 1, 2008; 104(1): 296 - 305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Duhamel, R. D. Stewart, A. R. Tupling, J. Ouyang, and H. J. Green Muscle sarcoplasmic reticulum calcium regulation in humans during consecutive days of exercise and recovery J Appl Physiol, October 1, 2007; 103(4): 1212 - 1220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. McKenna, I. Medved, C. A. Goodman, M. J. Brown, A. R. Bjorksten, K. T. Murphy, A. C. Petersen, S. Sostaric, and X. Gong N-acetylcysteine attenuates the decline in muscle Na+,K+-pump activity and delays fatigue during prolonged exercise in humans J. Physiol., October 1, 2006; 576(1): 279 - 288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Duhamel, H. J. Green, J. G. Perco, and J. Ouyang Effects of prior exercise and a low-carbohydrate diet on muscle sarcoplasmic reticulum function during cycling in women J Appl Physiol, September 1, 2006; 101(3): 695 - 706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Sostaric, t. l. S. L. Skinner, M. J. Brown, T. Sangkabutra, I. Medved, T. Medley, S. E. Selig, I. Fairweather, D. Rutar, and M. J. McKenna Alkalosis increases muscle K+ release, but lowers plasma [K+] and delays fatigue during dynamic forearm exercise J. Physiol., January 1, 2006; 570(1): 185 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-M. S. Thorud, E. Verburg, P. K. Lunde, T. A. Stromme, I. Sjaastad, and O. M. Sejersted Temperature-dependent skeletal muscle dysfunction in rats with congestive heart failure J Appl Physiol, October 1, 2005; 99(4): 1500 - 1507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. P. Holloway, H. J. Green, T. A. Duhamel, S. Ferth, J. W. Moule, J. Ouyang, and A. R. Tupling Muscle sarcoplasmic reticulum Ca2+ cycling adaptations during 16 h of heavy intermittent cycle exercise J Appl Physiol, September 1, 2005; 99(3): 836 - 843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.M. Meyers and E. Cafarelli Caffeine increases time to fatigue by maintaining force and not by altering firing rates during submaximal isometric contractions J Appl Physiol, September 1, 2005; 99(3): 1056 - 1063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Leppik, R. J. Aughey, I. Medved, I. Fairweather, M. F. Carey, and M. J. McKenna Prolonged exercise to fatigue in humans impairs skeletal muscle Na+-K+-ATPase activity, sarcoplasmic reticulum Ca2+ release, and Ca2+ uptake J Appl Physiol, October 1, 2004; 97(4): 1414 - 1423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Medved, M. J. Brown, A. R. Bjorksten, K. T. Murphy, A. C. Petersen, S. Sostaric, X. Gong, and M. J. McKenna N-acetylcysteine enhances muscle cysteine and glutathione availability and attenuates fatigue during prolonged exercise in endurance-trained individuals J Appl Physiol, October 1, 2004; 97(4): 1477 - 1485. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Duhamel, H. J. Green, J. G. Perco, S. D. Sandiford, and J. Ouyang Human muscle sarcoplasmic reticulum function during submaximal exercise in normoxia and hypoxia J Appl Physiol, July 1, 2004; 97(1): 180 - 187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Duhamel, H. J. Green, S. D. Sandiford, J. G. Perco, and J. Ouyang Effects of progressive exercise and hypoxia on human muscle sarcoplasmic reticulum function J Appl Physiol, July 1, 2004; 97(1): 188 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. T. Murphy, R. J. Snow, A. C. Petersen, R. M. Murphy, J. Mollica, J. S. Lee, A. P. Garnham, R. J. Aughey, J. A. Leppik, I. Medved, et al. Intense exercise up-regulates Na+,K+-ATPase isoform mRNA, but not protein expression in human skeletal muscle J. Physiol., April 15, 2004; 556(2): 507 - 519. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Medved, M. J. Brown, A. R. Bjorksten, and M. J. McKenna Effects of intravenous N-acetylcysteine infusion on time to fatigue and potassium regulation during prolonged cycling exercise J Appl Physiol, January 1, 2004; 96(1): 211 - 217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. McKenna, S. F. Fraser, J. L. Li, X. N. Wang, M. F. Carey, E. A. Side, J. Morton, G. I. Snell, K. Kjeldsen, and T. J. Williams Impaired muscle Ca2+ and K+ regulation contribute to poor exercise performance post-lung transplantation J Appl Physiol, October 1, 2003; 95(4): 1606 - 1616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Tupling, H. J. Green, B. D. Roy, S. Grant, and J. Ouyang Paradoxical effects of prior activity on human sarcoplasmic reticulum Ca2+-ATPase response to exercise J Appl Physiol, July 1, 2003; 95(1): 138 - 144. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. J. Green, C. S. Ballantyne, J. D. MacDougall, M. A. Tarnopolsky, and J. D. Schertzer Adaptations in human muscle sarcoplasmic reticulum to prolonged submaximal training J Appl Physiol, May 1, 2003; 94(5): 2034 - 2042. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Medved, M. J. Brown, A. R. Bjorksten, J. A. Leppik, S. Sostaric, and M. J. McKenna N-acetylcysteine infusion alters blood redox status but not time to fatigue during intense exercise in humans J Appl Physiol, April 1, 2003; 94(4): 1572 - 1582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. Fraser, J. L. Li, M. F. Carey, X. N. Wang, T. Sangkabutra, S. Sostaric, S. E. Selig, K. Kjeldsen, and M. J. McKenna Fatigue depresses maximal in vitro skeletal muscle Na+-K+-ATPase activity in untrained and trained individuals J Appl Physiol, November 1, 2002; 93(5): 1650 - 1659. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
