Journal of Applied Physiology Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 92: 1285-1292, 2002. First published November 30, 2001; doi:10.1152/japplphysiol.01009.2001
8750-7587/02 $5.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
92/3/1285    most recent
01009.2001v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hunt, D. G.
Right arrow Articles by Ivy, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hunt, D. G.
Right arrow Articles by Ivy, J. L.
Vol. 92, Issue 3, 1285-1292, March 2002

Clenbuterol prevents epinephrine from antagonizing insulin-stimulated muscle glucose uptake

Desmond G. Hunt, Zhenping Ding, and John L. Ivy

Exercise Physiology and Metabolism Laboratory, Department of Kinesiology and Health Education, University of Texas at Austin, Austin, Texas 78712

In the present study, we investigated the effects of chronic clenbuterol treatment on insulin-stimulated glucose uptake in the presence of epinephrine in isolated rat skeletal muscle. Insulin (50 µU/ml) increased glucose uptake in both fast-twitch (epitrochlearis) and slow-twitch (soleus) muscles. In the presence of 24 nM epinephrine, insulin-stimulated glucose uptake was completely suppressed. This suppression of glucose uptake by epinephrine was accompanied by an increase in the intracellular concentration of glucose 6-phosphate and a decrease in insulin-receptor substrate-1-associated phosphatidylinositol 3-kinase (IRS-1/PI3-kinase) activity. Clenbuterol treatment had no direct effect on insulin-stimulated glucose uptake. However, after clenbuterol treatment, epinephrine was ineffective in attenuating insulin-stimulated muscle glucose uptake. This ineffectiveness of epinephrine to suppress insulin-stimulated glucose uptake occurred in conjunction with its inability to increase the intracellular concentration of glucose 6-phosphate and attenuate IRS-1/PI3-kinase activity. Results of this study indicate that the effectiveness of epinephrine to inhibit insulin-stimulated glucose uptake is severely diminished in muscle from rats pretreated with clenbuterol.

glucose 6-phosphate; phosphatidylinositol 3-kinase; glycogen; beta -adrenergic receptors; propranolol


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. H. Mulder, C. J. Tack, A. J. Olthaar, P. Smits, F. C. G. J. Sweep, and R. R. Bosch
Adrenergic receptor stimulation attenuates insulin-stimulated glucose uptake in 3T3-L1 adipocytes by inhibiting GLUT4 translocation
Am J Physiol Endocrinol Metab, October 1, 2005; 289(4): E627 - E633.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. A. Ferreira, K. L. Eybrechts, A. I. M. Mocking, C. Kroner, and J.-W. N. Akkerman
IRS-1 Mediates Inhibition of Ca2+ Mobilization by Insulin via the Inhibitory G-protein Gi
J. Biol. Chem., January 30, 2004; 279(5): 3254 - 3264.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. G. Hunt and J. L. Ivy
Epinephrine inhibits insulin-stimulated muscle glucose transport
J Appl Physiol, November 1, 2002; 93(5): 1638 - 1643.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. G. Hunt, Z. Ding, and J. L. Ivy
Propranolol prevents epinephrine from limiting insulin-stimulated muscle glucose uptake during contraction
J Appl Physiol, August 1, 2002; 93(2): 697 - 704.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online