Spasticity, contracture and muscle weakness often occur together post stroke and cause considerable motor impairments to stroke survivors. The underlying changes in contractile properties of muscle fascicles are still not clear. The purpose of this study was to investigate the contractile property changes of the medial gastrocnemius muscle fascicles post stroke. Ten stroke survivors and 10 healthy subjects participated in the study. The medial gastrocnemius fascicular length was measured at various combinations of ankle and knee positions using ultrasonography, with the muscle activated selectively using electrical stimulation. The stimulation intensity was kept constant across different ankle and knee positions to establish the active force-length relationship of the muscle fascicles. It was found that stroke survivors showed a shift of the force-length curve with a significantly shorter optimal fascicle length (33.2±3.2 mm) compared to that of healthy controls (47.4±2.7 mm) with p<0.001. Furthermore, the width span of the fascicular force-length curve of stroke survivors was significantly narrower with steeper slopes than that of controls (p0.001), suggesting reduced number of sarcomeres along the fascicles and/or reduced sarcomere length post stroke. Regression analysis showed that the medial gastrocnemius fascicular length of stroke survivors varied significantly less with ankle and knee flexions (p0.001) than that of controls, suggesting shorter and stiffer muscle fascicles post stroke which might be attributed to muscle architectural adaptation. This study showed that there are considerable changes in the contractile properties of muscle fascicles post stroke which may contribute directly to the joint-level changes of decreased range of motion, increased stiffness, muscle weakness, and impaired motor functions in stroke survivors.