The limb-specific effects of aging upon vessel structure and function are not well understood. Consequently, in twelve young (26 ± 2 yrs) and twelve old (72 ± 1 yrs) healthy subjects we utilized ultrasound Doppler to evaluate intima-media thickness (IMT), ischemic reperfusion (IR), and flow-mediated dilation (FMD) following (5 min) suprasystolic cuff occlusion in both the arm (brachial artery (BA)) and the leg (popliteal artery (PA)). Structural measurements, whether normalized for vessel size or not, revealed a greater IMT in the both the BA and PA with age (young: BA: 0.028 ± 0.001 and PA: 0.046 ± 0.003, old: BA: 0.039 ± 0.002 and PA: 0.073 ± 0.005 cm; P < 0.05). IR revealed a similar pattern as IMT in terms of limb and age-related differences. There was an age-related attenuation in both BA FMD (old: 38% smaller BA FMD compared to young) and PA FMD (old: 71% smaller PA FMD compared to young). However, when this % change was normalized for shear rate, only the PA FMD of the old group was still significantly attenuated (old: 41% smaller PA FMD/shear rate compared to young). Together, the finding of differential structural and functional parameters in the arms and legs of healthy young people, and the somewhat negative findings that are specific to the legs of otherwise healthy older people (greater IMT and attenuated FMD), support and may help to better understand the increased propensity to develop a vascular pathology in the legs with age.