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Resistance exercise increases human skeletal muscle AS160/TBC1D4 phosphorylation in association with enhanced leg glucose uptake during postexercise recovery

¹Departments of Physical Therapy, ²Internal Medicine, and ³Surgery, and ⁴Division of Rehabilitation Sciences, University of Texas Medical Branch, Galveston, Texas

Submitted 23 April 2008 ; accepted in final form 11 September 2008

Akt substrate of 160 kDa (AS160/TBC1D4) is associated with insulin and contraction-mediated glucose uptake. Human skeletal muscle AS160 phosphorylation is increased during aerobic exercise but not immediately following resistance exercise. It is not known whether AS160 phosphorylation is altered during recovery from resistance exercise. Therefore, we hypothesized that muscle AS160/TBC1D4 phosphorylation and glucose uptake across the leg would be increased during recovery following resistance exercise. We studied 9 male subjects before, during, and for 2 h of postexercise recovery. We utilized femoral catheterizations and muscle biopsies in combination with indirect calorimetry and immunoblotting to determine whole body glucose and fat oxidation, leg glucose uptake, muscle AMPK2 activity, and the phosphorylation of muscle Akt and AS160/TBC1D4. Glucose oxidation was reduced while fat oxidation increased (35%) during postexercise recovery (P 0.05). Glucose uptake increased during exercise and postexercise recovery (P 0.05). Akt phosphorylation was increased at 1 h and AMPK2 activity increased at 2 h postexercise (P 0.05). Phospho(Ser/Thr)-Akt substrate (PAS) phosphorylation (often used as a marker for AS160) was unchanged immediately postexercise and increased at 1 h (P 0.05) and 2 h postexercise (P = 0.07). The PAS antibody is not always specific for AS160/TBC1D4 and can detect proteins at a similar molecular weight. Therefore, we immunoprecipitated AS160/TBC1D4 and then blotted with the PAS antibody, which confirmed that PAS phosphorylation is occurring on AS160/TBC1D4. There was also a positive correlation between PAS phosphorylation and leg glucose uptake during recovery (P < 0.05). We conclude that resistance exercise increases AS160/TBC1D4 phosphorylation in association with an increase in leg glucose uptake during postexercise recovery.

AMP-activated protein kinase; fat oxidation; GLUT4; Akt

This article has been cited by other articles:

				K. Funai, G. G. Schweitzer, N. Sharma, M. Kanzaki, and G. D. Cartee Increased AS160 phosphorylation, but not TBC1D1 phosphorylation, with increased postexercise insulin sensitivity in rat skeletal muscle Am J Physiol Endocrinol Metab, July 1, 2009; 297(1): E242 - E251. [Abstract] [Full Text] [PDF]