Journal of Applied Physiology Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 104: 1202-1210, 2008. First published January 24, 2008; doi:10.1152/japplphysiol.01160.2007
8750-7587/08 $8.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
104/4/1202    most recent
01160.2007v2
01160.2007v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (3)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Guenette, J. A.
Right arrow Articles by Boushel, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guenette, J. A.
Right arrow Articles by Boushel, R.

INNOVATIVE METHODOLOGY

Human respiratory muscle blood flow measured by near-infrared spectroscopy and indocyanine green

Jordan A. Guenette,1 Ioannis Vogiatzis,2,3 Spyros Zakynthinos,2 Dimitrios Athanasopoulos,2,3 Maria Koskolou,3 Spyretta Golemati,2 Maroula Vasilopoulou,2 Harrieth E. Wagner,4 Charis Roussos,2 Peter D. Wagner,4 and Robert Boushel5,6

1School of Human Kinetics, The University of British Columbia, Vancouver, British Columbia, Canada; 2Department of Critical Care Medicine and Pulmonary Services, Evangelismos Hospital, "M. Simou and G. P. Livanos Laboratories," National and Kapodistrian University of Athens, Athens, Greece; 3Department of Physical Education and Sport Sciences, National and Kapodistrian University of Athens, Athens, Greece; 4Department of Medicine, University of California-San Diego, La Jolla, California; 5Department of Exercise Science, Concordia University, Montreal, Quebec, Canada; and 6Department of Biomedical Sciences, Panum Institute, University of Copenhagen, Copenhagen, Denmark

Submitted 30 October 2007 ; accepted in final form 17 January 2008

Measurement of respiratory muscle blood flow (RMBF) in humans has important implications for understanding patterns of blood flow distribution during exercise in healthy individuals and those with chronic disease. Previous studies examining RMBF in humans have required invasive methods on anesthetized subjects. To assess RMBF in awake subjects, we applied an indicator-dilution method using near-infrared spectroscopy (NIRS) and the light-absorbing tracer indocyanine green dye (ICG). NIRS optodes were placed on the left seventh intercostal space at the apposition of the costal diaphragm and on an inactive control muscle (vastus lateralis). The primary respiratory muscles within view of the NIRS optodes include the internal and external intercostals. Intravenous bolus injection of ICG allowed for cardiac output (by the conventional dye-dilution method with arterial sampling), RMBF, and vastus lateralis blood flow to be quantified simultaneously. Esophageal and gastric pressures were also measured to calculate the work of breathing and transdiaphragmatic pressure. Measurements were obtained in five conscious humans during both resting breathing and three separate 5-min bouts of constant isocapnic hyperpnea at 27.1 ± 3.2, 56.0 ± 6.1, and 75.9 ± 5.7% of maximum minute ventilation as determined on a previous maximal exercise test. RMBF progressively increased (9.9 ± 0.6, 14.8 ± 2.7, 29.9 ± 5.8, and 50.1 ± 12.5 ml·100 ml–1·min–1, respectively) with increasing levels of ventilation while blood flow to the inactive control muscle remained constant (10.4 ± 1.4, 8.7 ± 0.7, 12.9 ± 1.7, and 12.2 ± 1.8 ml·100 ml–1·min–1, respectively). As ventilation rose, RMBF was closely and significantly correlated with 1) cardiac output (r = 0.994, P = 0.006), 2) the work of breathing (r = 0.995, P = 0.005), and 3) transdiaphragmatic pressure (r = 0.998, P = 0.002). These data suggest that the NIRS-ICG technique provides a feasible and sensitive index of RMBF at different levels of ventilation in humans.

work of breathing; ventilation; skeletal muscle blood flow


Address for reprint requests and other correspondence: J. A. Guenette, Health and Integrative Physiology Laboratory, Univ. of British Columbia, 6108 Thunderbird Blvd., Vancouver, BC, Canada V6T-1Z3




This article has been cited by other articles:


Home page
 J. Physiol.Home page
I. Vogiatzis, D. Athanasopoulos, H. Habazettl, W. M. Kuebler, H. Wagner, C. Roussos, P. D. Wagner, and S. Zakynthinos
Intercostal muscle blood flow limitation in athletes during maximal exercise
J. Physiol., July 15, 2009; 587(14): 3665 - 3677.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
I. Vogiatzis, D. Athanasopoulos, R. Boushel, J. A. Guenette, M. Koskolou, M. Vasilopoulou, H. Wagner, C. Roussos, P. D. Wagner, and S. Zakynthinos
Contribution of respiratory muscle blood flow to exercise-induced diaphragmatic fatigue in trained cyclists
J. Physiol., November 15, 2008; 586(22): 5575 - 5587.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
W. M. Kuebler
How NIR is the future in blood flow monitoring?
J Appl Physiol, April 1, 2008; 104(4): 905 - 906.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2008 by the American Physiological Society.