HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free All Versions of this Article: 104/3/773    most recent 00482.2007v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Emami, K. Articles by Rizi, R. R. Search for Related Content PubMed PubMed Citation Articles by Emami, K. Articles by Rizi, R. R.

Early changes of lung function and structure in an elastase model of emphysema—a hyperpolarized ³He MRI study

¹Department of Radiology and ²Division of Thoracic Surgery, University of Pennsylvania, Philadelphia, Pennsylvania; ³Department of Otolaryngology, Johns Hopkins University, Baltimore, Maryland; and ⁴Veterans Affairs Medical Center Hospital, Philadelphia, Pennsylvania

Submitted 4 May 2007 ; accepted in final form 4 December 2007

Early changes of lung function and structure were studied in the presence of an elastase-induced model of emphysema in 35 Sprague-Dawley rats at mild (5 U/100 g) and moderate (10 U/100 g) severities. Lung ventilation was measured on a regional basis (at a planar resolution of 3.2 mm) by hyperpolarized ³He MRI at 5 and 10 wk after model induction. Subsequent to imaging, average alveolar diameter was measured from histological slices taken from the centers of each lobe. Changes of mean fractional ventilation, mean linear intercept, and intrasubject heterogeneity of ventilation were studied during disease progression. Mean fractional ventilation was significantly different between healthy controls (0.23 ± 0.04) and emphysematous animals at both time points in the 10-unit group (0.06 ± 0.02 and 0.12 ± 0.05, respectively). Changes in average alveolar diameter were not statistically observable until the 10th wk between healthy (37 ± 10 µm) and emphysematous rats (73 ± 25 and 95 ± 31 µm, for 5 and 10 units, respectively). Assessment of function-structure correlation suggested that the majority of the decline in fractional ventilation occurred in the first 5 wk, while enlargement of alveolar diameters appeared primarily between the 5th and 10th wk. A thresholding metric, based on the 20th percentile of fractional ventilation over the entire lung, was utilized to detect the onset of the disease with confidence, independent of whether the regional ventilation measurements were normalized with respect to the delivered tidal volume and estimated functional residual capacity of each individual rat.

lung physiology; early detection of emphysema; regional fractional ventilation; hyperpolarized helium-3 magnetic resonance imaging

This article has been cited by other articles:

				V. R. Muzykantov Chair's Summary Proceedings of the ATS, August 15, 2009; 6(5): 398 - 402. [Full Text] [PDF]

				K. Emami, M. Stephen, S. Kadlecek, R. V. Cadman, M. Ishii, and R. R. Rizi Quantitative Assessment of Lung Using Hyperpolarized Magnetic Resonance Imaging Proceedings of the ATS, August 15, 2009; 6(5): 431 - 438. [Abstract] [Full Text] [PDF]

				R. E. Jacob, K. R. Minard, G. Laicher, and C. Timchalk 3D 3He diffusion MRI as a local in vivo morphometric tool to evaluate emphysematous rat lungs J Appl Physiol, October 1, 2008; 105(4): 1291 - 1300. [Abstract] [Full Text] [PDF]