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Myocyte enlargement, differentiation, and proliferation kinetics in the fetal sheep heart

¹Department of Physiology and Pharmacology, ²Heart Research Center, and ³Department of Medicine (Cardiovascular Medicine), Oregon Health and Science University, and ⁴Portland VA Medical Center, Portland, Oregon; and ⁵Center for Perinatal Biology, Department of Pharmacology, Loma Linda University School of Medicine, Loma Linda, California

Submitted 23 August 2006 ; accepted in final form 16 November 2006

The generation of new myocytes is an essential process of in utero heart growth. Most, or all, cardiac myocytes lose their capacity for proliferation during the perinatal period through the process of terminal differentiation. An increasing number of studies focus on how experimental interventions affect cardiac myocyte growth in the fetal sheep. Nevertheless, fundamental questions about normal growth of the fetal heart remain unanswered. In this study, we determined that during the last third of gestation the hearts of fetal sheep grew primarily by four processes. 1) Myocyte proliferation contributed substantially to daily cardiac mass gain, and the number of cardiac myocytes continued to increase to term. 2) The (hitherto unrecognized) contribution to cardiac growth by the increase in myocyte size associated with the transition from mononucleation to binucleation (terminal differentiation) became considerable from 115 days of gestational age (dGA) until term (145dGA). Because binucleation became the more frequent outcome of myocyte cell cycle activity after 115dGA, the number of binucleated myocytes increased at the expense of the number of mononucleated myocytes. Both the interval between nuclear divisions and the duration of cell cycle activity in myocytes decreased substantially during this same period. Finally, cardiac growth was in part due to enlargement of 3) mononucleated and 4) binucleated myocytes, which grew in cross-sectional diameter but not length during the last third of gestation. These data on normal cardiac growth may enable a more detailed understanding of the consequences of experimental and pathological interventions in prenatal life.

hyperplasia; hypertrophy; terminal differentiation; apoptosis

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