HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free All Versions of this Article: 101/6/1648    most recent 00394.2006v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (13) Citing Articles via Google Scholar Google Scholar Articles by Wilson, D. F. Articles by Vinogradov, S. A. Search for Related Content PubMed PubMed Citation Articles by Wilson, D. F. Articles by Vinogradov, S. A.

Oxygen pressures in the interstitial space and their relationship to those in the blood plasma in resting skeletal muscle

Departments of ¹Biochemistry and Biophysics and ²Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

Submitted 3 April 2006 ; accepted in final form 24 July 2006

This study compared oxygen pressures (PO₂), measured by oxygen-dependent quenching of phosphorescence, in the intravascular (blood plasma) space in the muscle with those in the interstitial (pericellular) space. Our hypothesis was that the capillary wall would not significantly impede oxygen diffusion from the blood plasma to the pericellular space. A new near-infrared oxygen sensitive probe, Oxyphor G3, was used to obtain oxygen distributions in the interstitial space. Oxyphor G3 is a Pd-tetrabenzoporphyrin encapsulated inside generation 2 poly-arylglycine (AG) dendrimer. The periphery of the dendrimer is modified with oligoethylene glycol residues (average molecular weight 350) to make the probe water soluble and biologically inert. Oxyphor G3 was injected into thigh muscle using a 30-gauge needle. Histograms of the PO₂ in the interstitial space were measured in awake and anesthetized animals and compared with those for Oxyphor G2 in the intravascular (blood plasma) space. For awake mice, the lowest 10% of PO₂ values for the interstitial and intravascular spaces (believed to represent capillary bed) were not significantly different [23.8 (SD 4.5) and 25 Torr (SD 4.3), respectively], whereas, in isoflurane-anesthetized mice, there was a small but significant (P = 0.01) difference [20.4 (SD 6.3) and 27.9 Torr (SD 3.5), respectively]. The peak values for the histograms for the interstitial space in awake and isoflurane-anesthetized mice were 40.8 (SD 7.5) and 36.9 Torr (SD 8.3), respectively, whereas those for the intravascular space were 52.2 (SD 4.9) and 55.9 Torr (SD 8.4), respectively, showing no significant difference due to isoflurane anesthesia. The histograms for the intravascular space were significantly wider, with more contribution at higher PO₂ values. A different anesthetic, ketamine plus xylazine injected intraperitoneally, caused a marked decrease in the tissue PO₂ values in both spaces, with the time course and extent of the decrease dependent on the time after injection and variable among mice. It was, therefore, not further used.

phosphor; phosphorescence; phosphorescence quenching; oxygen histograms; anesthetics; rodent; tissue oxygenation

This article has been cited by other articles:

				E. G. Mik, T. Johannes, C. J. Zuurbier, A. Heinen, J. H. P. M. Houben-Weerts, G. M. Balestra, J. Stap, J. F. Beek, and C. Ince In Vivo Mitochondrial Oxygen Tension Measured by a Delayed Fluorescence Lifetime Technique Biophys. J., October 15, 2008; 95(8): 3977 - 3990. [Abstract] [Full Text] [PDF]

				A. S. Golub and R. N. Pittman PO2 measurements in the microcirculation using phosphorescence quenching microscopy at high magnification Am J Physiol Heart Circ Physiol, June 1, 2008; 294(6): H2905 - H2916. [Abstract] [Full Text] [PDF]

				M. A. Robinson, J. E. Baumgardner, V. P. Good, and C. M. Otto Physiological and hypoxic O2 tensions rapidly regulate NO production by stimulated macrophages Am J Physiol Cell Physiol, April 1, 2008; 294(4): C1079 - C1087. [Abstract] [Full Text] [PDF]

				E. G. Mik, T. Johannes, and C. Ince Monitoring of renal venous PO2 and kidney oxygen consumption in rats by a near-infrared phosphorescence lifetime technique Am J Physiol Renal Physiol, March 1, 2008; 294(3): F676 - F681. [Abstract] [Full Text] [PDF]

				A. S. Golub, M. C. Barker, and R. N. Pittman Microvascular oxygen tension in the rat mesentery Am J Physiol Heart Circ Physiol, January 1, 2008; 294(1): H21 - H28. [Abstract] [Full Text] [PDF]

				D. F. Wilson Quantifying the role of oxygen pressure in tissue function Am J Physiol Heart Circ Physiol, January 1, 2008; 294(1): H11 - H13. [Full Text] [PDF]

				A. S. Golub, M. C. Barker, and R. N. Pittman PO2 profiles near arterioles and tissue oxygen consumption in rat mesentery Am J Physiol Heart Circ Physiol, August 1, 2007; 293(2): H1097 - H1106. [Abstract] [Full Text] [PDF]

				A. G. Tsai, P. Cabrales, P. C. Johnson, and M. Intaglietta New phosphorescence quenching oxygen measurements technique yields unusual tissue and plasma PO2 distributions J Appl Physiol, May 1, 2007; 102(5): 2081 - 2082. [Full Text] [PDF]

				D. F. Wilson, W. M. F. Lee, S. Makonnen, O. Finikova, S. Apreleva, and S. A. Vinogradov Reply to Tsai, Cabrales, Johnson, and Intaglietta J Appl Physiol, May 1, 2007; 102(5): 2083 - 2083. [Full Text] [PDF]