Journal of Applied Physiology Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Appl Physiol 101: 785-788, 2006. First published May 25, 2006; doi:10.1152/japplphysiol.00135.2006
8750-7587/06 $8.00
This Article
Right arrow Full Text Free
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
101/3/785    most recent
00135.2006v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (5)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mills, P. J.
Right arrow Articles by Maisel, A. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mills, P. J.
Right arrow Articles by Maisel, A. S.

Physical fitness attenuates leukocyte-endothelial adhesion in response to acute exercise

Paul J. Mills,1 Suzi Hong,1 Laura Redwine,2 Steven M. Carter,2 Albert Chiu,2 Michael G. Ziegler,2 Joel E. Dimsdale,1 and Alan S. Maisel2

Departments of 1Psychiatry and 2Medicine, University of California, San Diego, La Jolla, California

Submitted 2 February 2006 ; accepted in final form 8 May 2006

Studies suggest that physical fitness promotes cardiovascular health, including improved endothelial function and possibly reduced inflammatory responses to stressors. This study examined the effects of fitness on leukocyte-endothelial adhesion in response to an acute exercise challenge. Peripheral blood mononuclear cell (PBMC) adhesion to human umbilical venous endothelial cells (HUVEC) was examined in 18 more-fit and 19 less-fit individuals [mean age 39 yr (SD 11)] before and after a 20-min treadmill exercise at 65–70% peak oxygen consumption. PBMC were isolated from whole blood (Ficoll-Paque) at rest and immediately after exercise. HUVEC were incubated for 4 h in the presence of cytokines IL-1 and IL-8 to activate endothelial adhesion molecule expression. Fit subjects showed a significant reduction in PBMC-HUVEC adhesion after exercise (P < 0.01) compared with less-fit subjects, who showed no significant change. Regardless of fitness levels, both at rest and in response to exercise, soluble ICAM-1 in the incubation media attenuated PBMC-HUVEC adhesion by ~81% (P < 0.001). The findings indicate that immune cells that demarginate in response to exercise have reduced ability to adhere in individuals who are physically fit, an effect apparently independent of ICAM-1 binding. The findings provide evidence of how physical fitness might protect individuals from inflammatory responses to exercise.

endothelium; adhesion; soluble intercellular adhesion molecule-1; peripheral blood mononuclear cell


Address for reprint requests and other correspondence: P. J. Mills, UCSD Medical Center, 200 West Arbor Dr., San Diego, CA 92103-0804 (e-mail: pmills{at}ucsd.edu)




This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
D. Markovitch, R. M. Tyrrell, and D. Thompson
Acute moderate-intensity exercise in middle-aged men has neither an anti- nor proinflammatory effect
J Appl Physiol, July 1, 2008; 105(1): 260 - 265.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2006 by the American Physiological Society.