HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Free Full Text (PDF) Free All Versions of this Article: 100/5/1452    most recent 00997.2005v1 Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by Zhang, P. Articles by Yokota, H. Search for Related Content PubMed PubMed Citation Articles by Zhang, P. Articles by Yokota, H.

Diaphyseal bone formation in murine tibiae in response to knee loading

¹Departments of Anatomy and Cell Biology, and ³Biomedical Engineering, Indiana University-Purdue University Indianapolis, Indianapolis, Indiana; and ²Graduate School of Natural Science and Technology, Kanazawa University, Ishikawa, Japan

Submitted 18 August 2005 ; accepted in final form 30 December 2005

Mechanical stimulation is critical for bone architecture and bone mass. The aim of this study was to examine the effects of mechanical loads applied to the knee. The specific question was whether loads applied to the tibial epiphysis would enhance bone formation in the tibial diaphysis. In C57/BL/6 mice, loads of 0.5 N were applied for 3 min per day for 3 days at 5, 10, or 15 Hz. Bone samples were harvested 13 days after the last loading. The strains were measured 13 ± 2 µstrains at 5 Hz in the diaphysis. The histomorphometric data in the diaphysis clearly showed enhanced bone formation. First, compared with nonloaded control the cross-sectional cortical area was increased by 11% at 5 Hz and 8% at 10 Hz (both P < 0.05). Second, the cortical thickness was elevated by 12% at 5 Hz (P < 0.01) and 8% at 10 Hz (P < 0.05). Third, mineralizing surface (MS/BS), mineral apposition rate (MAR), and bone formation rate (BFR/BS) were increased at 5 Hz (P < 0.01 for MS/BS; P < 0.001 for MAR and BFR/BS) and at 10 Hz (P < 0.05 for MS/BS; P < 0.01 for MAR and BFR/BS). Bone formation was enhanced more extensively in the medial side than the lateral or the posterior side. The results reveal that knee loading is an effective means to enhance bone formation in the tibial diaphysis in a loading-frequency dependent manner without inducing significant in situ strain at the site of bone formation.

mechanical loading of bone; mouse; tibial diaphysis

This article has been cited by other articles:

				P Zhang, G M Malacinski, and H Yokota Joint loading modality: its application to bone formation and fracture healing Br. J. Sports Med., July 1, 2008; 42(7): 556 - 560. [Abstract] [Full Text] [PDF]

				S. J. Warden Breaking the rules for bone adaptation to mechanical loading J Appl Physiol, May 1, 2006; 100(5): 1441 - 1442. [Full Text] [PDF]