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In vivo inhibition of nitric oxide synthase impairs upregulation of contractile protein mRNA in overloaded plantaris muscle

Center for Exercise Science, University of Florida, Gainesville, Florida

Submitted 1 August 2005 ; accepted in final form 14 September 2005

Inhibition of nitric oxide synthase (NOS) activity in vivo impedes hypertrophy in the overloaded rat plantaris. We investigated the mechanism for this effect by examining early events leading to muscle growth following 5 or 12 days of functional overload. Male Sprague-Dawley rats (350 g) were randomly divided into three treatment groups: control, N^G-nitro-L-arginine methyl ester (L-NAME; 90 mg·kg^–1·day^–1), and 1-(2-trifluoromethyl-phenyl)-imidazole (TRIM; 10 mg·kg^–1·day^–1). Unilateral removal of synergists induced chronic overload (OL) of the right plantaris. Sham surgery performed on the left hindlimb served as a normally loaded control. L-NAME and TRIM treatments prevented OL-induced skeletal -actin and type I (slow) myosin heavy chain mRNA expression at 5 days. Conversely, neither L-NAME nor TRIM affected hepatocyte growth factor or VEGF mRNA responses to OL at 5 days. However, OL induction of IGF-I and mechanogrowth factor mRNA was greater (P < 0.05) in the TRIM group compared with the controls. Furthermore, the phosphorylated-to-total p70 S6 kinase ratio was higher in OL muscle from NOS-inhibited groups, compared with control OL. At 12 days of OL, the cumulative proliferation of plantaris satellite cells was assessed by subcutaneous implantation of time release 5'-bromo-2'-deoxyuridine pellets during the OL-inducing surgeries. Although OL caused a fivefold increase in the number of mitotically active (5'-bromo-2'-deoxyuridine positive) sublaminar nuclei, this was unaffected by concurrent NOS inhibition. Therefore, NOS activity may provide negative feedback control of IGF-I/p70 S6 kinase signaling during muscle growth. Moreover, NOS activity may be involved in transcriptional regulation of skeletal -actin and type I (slow) myosin heavy chain during functional overload.

insulin-like growth factor-I; p70 S6 kinase; functional overload; 1-(2-trifluoromethyl-phenyl)-imidazole; N^G-nitro-L-arginine methyl ester

This article has been cited by other articles:

				J. A. Drenning, V. A. Lira, C. G. Simmons, Q. A. Soltow, J. E. Sellman, and D. S. Criswell Nitric oxide facilitates NFAT-dependent transcription in mouse myotubes Am J Physiol Cell Physiol, April 1, 2008; 294(4): C1088 - C1095. [Abstract] [Full Text] [PDF]