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1 Human Performance Laboratory, Ball State University, Muncie, Indiana, United States
* To whom correspondence should be addressed. E-mail: strappe{at}bsu.edu.
The purpose of this study was to investigate mRNA expression of several key skeletal muscle myogenic controllers; myogenic differentiation factor (MyoD), muscle regulatory factor 4 (MRF4), myogenic factor 5 (Myf5), Myogenin, Myostatin, and myocyte enhancer factor 2 (MEF2) at rest and 4 hrs after a single bout of resistance exercise (RE) in young and old women. Eight young women (YW) (23±2 y, 67±5 kg) and 6 old women (OW) (85±1 y, 67±4 kg) performed 3 sets of 10 repetitions of bilateral knee extensions at 70% of 1-repetition maximum. Muscle biopsies were taken from the vastus lateralis before and 4 hrs after RE. Using real-time RT PCR, mRNA from the muscle samples was amplified and normalized to GAPDH. At rest, OW expressed higher (p<0.05) levels of MyoD, MRF4, Myf5, Myogenin, and Myostatin compared to YW. In response to RE, there was a main time-effect (p<0.05) for the YW and OW combined in the up regulation of MyoD (2.0 fold), MRF4 (1.4-fold), and in the down regulation of Myostatin (2.2-fold). There was a trend (p=0.08) for time x age interaction in MRF4. These data show that old women express higher myogenic mRNA levels at rest. The higher resting myogenic mRNA levels in old women may reflect an attempt to preserve muscle mass and function. When challenged with RE, old women appear to respond in a similar manner as young women.
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