To examine the influence of exercise intensity on the increases in vastus lateralis GLUT-4 mRNA and protein following exercise, six untrained men exercised for 60 min at 39 ± 3% VO2 peak (LO) or 27 ± 2 min at 83 ± 2% VO2 peak (HI) in counterbalanced order. Pre-exercise muscle glycogen levels were not different between trials (LO: 408 ± 35 mmol.kg-1 dry mass; HI: 420 ± 43); however, post exercise levels were lower (P<0.05) in HI (169 ± 18) compared with LO (262 ± 35). Thus, calculated muscle glycogen utilisation was greater (P<0.05) in HI (251 ± 24 mmol. kg-1) than in LO (146 ± 34). Exercise resulted in similar increases in GLUT-4 gene expression in both trials. GLUT-4 mRNA was increased immediately at the end of exercise (~2-fold, P<0.05) and remained elevated after 3 hr of post-exercise recovery. When measured 3 hr after exercise, total crude membrane GLUT-4 protein levels were 106% higher in LO (3.3 ± 0.7 vs. 1.6 ± 0.3 arb. units) and 61% higher in HI (2.9 ± 0.5 vs. 1.8 ± 0.5) relative to pre-exercise levels. A main effect for exercise was observed, with no significant differences between trials. In conclusion, exercise at ~40 and ~80% VO2 peak, with total work equal, increased GLUT-4 mRNA and GLUT-4 protein in human skeletal muscle to a similar extent, despite differences in exercise intensity and duration.