A single session of exercise increases insulin sensitivity for hours and even days and dietary carbohydrate ingested after exercise alters the magnitude and duration of this effect. Although increasing systemic fatty acid availability is associated with insulin resistance, it is uncertain if increasing dietary fat availability after exercise alters the exercise-induced increase in insulin sensitivity. The purpose of this study was to determine if adding fat calories to meals after exercise alters insulin sensitivity the next day. Seven healthy men cycled 90 minutes at 66 ± 2% VO_2peak followed by a maximum of five high intensity intervals. During the hours after exercise, subjects ingested 3 meals containing either LOW-FAT (5% energy from fat) or HIGH-FAT (45% energy from fat) foods. Each diet contained the same amount of carbohydrate and protein. An oral glucose tolerance test (OGTT) was performed the next morning. Muscle glycogen and intramuscular triglyceride (IMTG) concentrations were measured in muscle biopsy samples obtained immediately before exercise and the next morning. The day after exercise, muscle glycogen concentration was identical in HIGH-FAT and LOW-FAT (393 ± 70 and 379 ± 38 mmol/kg dry weight). At the same time, IMTG concentration was ~20% greater during HIGH-FAT compared with LOW-FAT (42.5±3.4 and 36.3±3.3 mmol/kg dry weight; P<0.05). Despite the addition of [165g of fat to meals after exercise (~1500 kcal) and a resultant elevation in IMTG concentration, glucose tolerance was identical in HIGH-FAT and LOW-FAT (Composite index: 8.7±1.0 and 8.4±1.0). In summary, as long as meals ingested in the hours after exercise contain the same carbohydrate content, the addition of ~1500 kcal from fat to these meals did not alter muscle glycogen resynthesis or glucose tolerance the next day.