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J Appl Physiol (January 16, 2004). doi:10.1152/japplphysiol.01273.2003
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Submitted on December 1, 2003
Accepted on January 8, 2004

Inactivation of human muscle Na+-K+-ATPase in vitro during prolonged exercise is increased with hypoxia

Shelley D Sandiford, Howard J Green*, Todd A Duhamel, Jennifer G Perco, Jonathan D Schertzer, and Jing Ouyang

* To whom correspondence should be addressed. E-mail: green{at}healthy.uwaterloo.ca.

This study investigated the effects of prolonged exercise performed in normoxia (N) and hypoxia (H) on neuromuscular fatigue, membrane excitability and Na+-K+-ATPase activity in working muscle. Ten untrained volunteers (VO2peak=42.1±2.8 ml/kg/min; x±SE) performed 90 min of cycling during N (FIO2=0.21) and during H (FIO2=0.14) as approximately 50% of normoxic VO2peak. During N, 3-0-methylfluorescein phosphatase (3-0-MFPase) activity (nmol.mg protein-1.h-1) in vastus lateralis, used as a measure of Na+-K+-ATPase activity, decreased (P<0.05) by 21% at 30 min of exercise compared to rest (101±53 vs 79.6±4.3) with no further reductions observed at 90 min (72.8±8.0). During H, similar reductions (P<0.05) were observed during the first 30 min (90.8±5.3 vs 79.0±6.3) followed by further reductions (P<0.05) at 90 min (50.5±3.9). Exercise in N resulted in reductions (P<0.05) in both in quadriceps maximal voluntary contractile force (MVC; 633±50 vs 477±67 N.m) and force at low frequencies of stimulation, namely 10 Hz (142±16 vs 86.7±10 N) and 20 Hz (283±32 vs 236±31 N). No changes were observed in the amplitude, duration and area of the muscle compound action potential (M-wave). Exercise in H was without additional effect in altering MVC, low frequency force and M-wave properties. It is concluded that although exercise in H resulted in a greater inactivation of Na+-K+-ATPase activity compared to N, neuromuscular fatigue and membrane excitability are not differentially altered.




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