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1 1Heller Institute of Medical Research, Sheba Medical Center, Tel Hashomer, Israel
2 Laboratory of Environmental Physiology, The Hebrew University, Jerusalem, Israel
3 Department of Rehabilitation, Hadassah University Hospital Mt Scopus, Jerusalem, Israel
* To whom correspondence should be addressed. E-mail: horowitz{at}cc.huji.ac.il.
During exertion in the heat, Heat Intolerant (HI) subjects have a physiological disability in metabolic heat dissipation. The HI state is either permanent or temporary, depending on whether it stems from transient predisposing factors or inherent thermoregulatory dysfunction. In this investigation, we studied protein levels of HSP 70 and HSP72, HSP90, BCL-2 xl, GST-p, HSF1, TAF and NFkB transcripts using Western blot and qRT-PCR, respectively, in lymphocytes of HI and tolerant (T) male volunteers of similar anthropometric features. Measurements were made from blood drawn before, during heat tolerance test (3.5mph, 40°C, 40 % relative humidity (R.H.), 2 hrs), and 1 hour after recovery at 24°C. Rectal (Tre) and skin (Tsk) temperatures, as well as heart rate, were continuously recorded. Of 58 subjects, 7 were identified as HI, with a significantly higher PSI (physiological strain index) than in the T group (6.3±0.9 vs 3.8±0.6, p<0.001, respectively). The responsiveness of the vasculature to thermal stimuli was decreased in the HI group, as indicated by Tre-Tsk. The HSP 72 level in the HI group dropped during the recovery session (p<0.01), whereas that of the T group continued to rise. A significantly increased expression of the transcription factors in the T subjects and significantly decreased expression in the HI group (p<0.009, 0.013, 0.005 for HSF-1, NFkB and TAF, respectively) points to impaired transcriptional processes in the HI group. Our data suggest that transcriptional malfunction and sluggishness of the vasculature to thermal stimuli are predisposing factors in the HI Group.
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