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Articles in PresS, published online ahead of print July 12, 2002
J Appl Physiol, 10.1152/jap.01247.2001
Submitted on December 21, 2001
Accepted on July 11, 2002
1 School of Human Movement, Recreation and Performance, Victoria University of Technology, Melbourne, Victoria, Australia; School of Medical Sciences, RMIT University, Melbourne, Victoria, Australia
2 School of Human Movement, Recreation and Performance, Victoria University of Technology, Melbourne, Victoria, Australia
3 School of Life Science, Victoria University of Technology, Melbourne, Victoria, Australia
4 School of Human Movement, Recreation and Performance, Victoria University of Technology, Melbourne, Victoria, Australia; Faculty of Medicine, Thammasat University, Rangsit, Thailand
5 Dept of Medicine B, Rigshospitalet, Copenhagen, Denmark
* To whom correspondence should be addressed. E-mail: michael.mckenna{at}vu.edu.au.
This study investigated whether fatiguing dynamic exercise depresses maximal in vitro Na+,K+ATPase activity and whether any depression is attenuated with chronic training. Eight untrained (UT), eight resistance-trained (RT) and eight endurance-trained (ET) subjects performed a quadriceps fatigue test, comprising 50 maximal isokinetic contractions (180°/s, 0.5Hz). Muscle biopsies (v.lateralis) were taken before and immediately after exercise and analyzed for maximal in vitro Na+,K+ATPase (K+-stimulated 3-O-methylfluoroscein phosphatase, 3-O-MFPase) activity. Resting samples were analysed for [3H]-ouabain binding site content, which was 16.6% and 18.3% higher (P<0.05) in ET than RT and UT, respectively (UT 311±41, RT 302±52, ET 357±29 pmol.g ww-1). 3-O-MFPase activity was depressed at fatigue by -13.8±4.1% (P<0.05), with no differences between groups (UT -13±4, RT -9±6, ET -22±6%). During incremental exercise, ET had a lower 
[K+].work-1 ratio than UT (P<0.05) and tended (P=0.09) to be lower than RT (UT 18.5±2.3, RT 16.2±2.2, ET 11.8±0.4, nmol.l-1.J-1). In conclusion, maximal in vitro Na+,K+-ATPase activity was depressed with fatigue, regardless of training state, suggesting this may be an important determinant of fatigue.
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