| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA; Department of Mechanical Engineering and Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA, USA
2 Department of Mechanical Engineering and Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA, USA
* To whom correspondence should be addressed. E-mail: rdkamm{at}mit.edu.
Neutrophils traversing the pulmonary microcirculation are subjected to mechanical stimulation during their deformation into narrow capillaries. To better understand the time-dependant changes caused by this mechanical stimulus, neutrophils were caused to flow into a microchannel, which allowed simultaneous visualization of cell morphology, and passive rheological measurement by tracking the Brownian motion of endogenous granules. Above a threshold stimulus, mechanical deformation resulted in neutrophil activation with pseudopod projection. The activation time was inversely correlated to the rate of mechanical deformation experienced by the neutrophils. A reduction in shear moduli was observed within seconds after the onset of the mechanical stimulus, suggesting a sudden disruption of the neutrophil cytoskeleton when subjected to mechanical deformation. However, the magnitude of the reduction in moduli was independent of the degree of deformation. Recovery to nearly the initial values of viscoelastic moduli occurred within one minute. These observations confirm that mechanical deformation of neutrophils, similar to conditions encountered in the pulmonary capillaries is not a passive event, rather, it is capable of activating the neutrophils and enhancing their migratory tendencies.
This article has been cited by other articles:
|
|
 |
|
  G. Popescu, Y. Park, N. Lue, C. Best-Popescu, L. Deflores, R. R. Dasari, M. S. Feld, and K. Badizadegan Optical imaging of cell mass and growth dynamics Am J Physiol Cell Physiol, August 1, 2008; 295(2): C538 - C544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Dangaria and P. J. Butler Macrorheology and adaptive microrheology of endothelial cells subjected to fluid shear stress Am J Physiol Cell Physiol, November 1, 2007; 293(5): C1568 - C1575. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Li, G. Lykotrafitis, M. Dao, and S. Suresh Cytoskeletal dynamics of human erythrocyte PNAS, March 20, 2007; 104(12): 4937 - 4942. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Yap and R. D. Kamm Cytoskeletal remodeling and cellular activation during deformation of neutrophils into narrow channels J Appl Physiol, December 1, 2005; 99(6): 2323 - 2330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Sieck J Appl Physiol, May 1, 2005; 98(5): 1891 - 1891. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
