Journal of Applied Physiology AJP: Cell Physiology
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J Appl Physiol (February 10, 2005). doi:10.1152/japplphysiol.01199.2004
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Submitted on October 25, 2004
Accepted on January 20, 2005

Intravital Microscopic Observations of 15 µm Microspheres Lodging in the Pulmonary Microcirculation

Wayne J.E. Lamm1, Susan L. Bernard1, Wiltz W. Wagner, Jr.2, and Robb W. Glenny3*

1 Department of Medicine, University of Washington, Seattle, WA, USA
2 Department of Anesthesiology, Indiana University School of Medicine, Indianapolis, IN, USA; Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Indianapolis, IN, USA; Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, USA
3 Department of Medicine, University of Washington, Seattle, WA, USA; Departments of Physiology and Biophysics, University of Wasington, Seattle, WA, USA

* To whom correspondence should be addressed. E-mail: glenny{at}u.washington.edu.

Vascular infusions of 15 µm-diameter microspheres are used to study pulmonary blood flow distribution. The sites of microsphere lodging and their effects on microvascular perfusion are debated but unknown. Using intravital microscopy of the subpleural surface of rat lungs, we directly observed deposition of fluorescent microspheres. In a pump-perfused lung model, ~ 0.5 million microspheres were infused over 30 s into the pulmonary artery of seven rats. Microsphere lodging was analyzed for the location in the microvasculature and the effect on local flow after lodging. On average, we observed 3.2 microspheres per 160 alveolar facets. The microspheres always entered the arterioles as singlets and lodged at the inlets to capillaries, either in alveolar corner vessels or small arterioles. In all cases, blood flow continued either around the microspheres or into the capillaries via adjacent pathways. We conclude that 15 µm-diameter microspheres, in doses in excess of those used in typical studies, have no significant impact on pulmonary capillary blood flow distribution.




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