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1 Human Performance Laboratory, Ball State University, Muncie, IN, USA
* To whom correspondence should be addressed. E-mail: strappe{at}bsu.edu.
The aim of this study was to examine the time course activation of select myogenic (MRF4, Myf5, MyoD, myogenin) and metabolic (CD36, CPT1, HK II and PDK4) genes after an acute bout of resistance (RE) or run (RUN) exercise. For RE, 6 subjects [25 ± 4 yr (mean ± SD), 74 ± 14 kg, 1.71 ± 0.11 m] performed 3 sets of 10 repetitions at 70% of 1 repetition maximum of bilateral knee extensions. For RUN, another 6 subjects (25 ± 4 yr, 72 ± 5 kg, 1.81 ± 0.07 m, 63 ± 8 ml.kg-1.min-1) performed a 30 min treadmill run at 75% of VO2max. All subjects were physically active in their respective mode of exercise. Eight muscle biopsies were taken from the vastus lateralis of RE subjects and from the gastrocnemius of RUN subjects pre, immediate post (0) and 1, 2, 4, 8, 12 and 24 h post exercise. RE increased mRNA of MRF4 (3.7- to 4.5-fold 2-4 h post), MyoD (5.8-fold 8 h post), myogenin (2.6- and 3.5-fold 8-12 h post), HKII (3.6- to 10.5-fold 2-12 h post) and PDK4 (14- to 26-fold 2-8 h post). There were no differences in Myf5, CD36 and CPT1 mRNA levels 0-24 h post RE. RUN increased mRNA of MyoD (5.0- to 8.0-fold), HKII (12- to 16-fold) and PDK4 (32- to 52-fold) at 8-12 h post exercise. There were no differences in MRF4, Myf5, myogenin, CD36 and CPT1 mRNA levels 0-24 h post RUN. These data show that there is both a myogenic and metabolic gene induction with RE and RUN exercise. The timing of the gene induction is variable and generally peaks 4-8 h post exercise with all gene expression not significantly different from the pre exercise levels by 24 h post exercise. These data provide basic information for the timing of human muscle biopsy samples for gene expression studies involving exercise.
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