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1 Integrative Physiology, University of Colorado, Boulder, Colorado, United States
2 United States; Department of Integrative Physiology, University of Colorado, Boulder, Colorado, United States
3 Department of Integrative Physiology, University of Colorado, Boulder, Colorado, United States
4 Medicine, UCHSC, 80262, Colorado, United States
* To whom correspondence should be addressed. E-mail: desouzac{at}colorado.edu.
Numerical and functional impairment of circulating endothelial progenitor cells (EPCs) are thought to contribute to vascular aging and the associated increase in cardiovascular risk. We tested the following hypotheses: 1) EPC clonogenic and migratory capacity decreases progressively with age in healthy, sedentary adult men; and 2) regular aerobic exercise will improve EPC clonogenic and migratory capacity in previously sedentary middle-aged and older men. Peripheral blood samples were collected from 46 healthy sedentary men: 10 young (26±1 yr), 15 middle-aged (47±1 yr) and 21 older (63±1 yr). Mononuclear cells were isolated, preplated for 2 days and nonadherent cells were further cultured for 7 days to determine EPC colony forming units. Migratory activity of EPCs was determined using a modified Boyden chamber. Ten sedentary middle-aged and older men (59±3 yr) were studied before and after a 3-month aerobic exercise intervention. The number of EPC colony forming units was ~75% lower (P<0.01) in middle-aged (12±3) and older (8±2) compared with young (40±7) men. There was no difference in colony count between middle-aged and older men. EPC migration (fluorescent units) was significantly reduced in older (452±72) compared with young (813±114) and middle-aged (760+114) men. The exercise intervention increased (P<0.05) both EPC colony forming units (10+3 to 22+5) and migratory activity (683±96 to 1022±123) in previously sedentary middle-aged and older men. These results provide further evidence that aging adversely affects EPC function. Regular aerobic-endurance exercise however, is an effective lifestyle intervention strategy for improving EPC clonogenic and migratory capacity in middle-aged and older healthy men.
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