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Articles in PresS, published online ahead of print May 10, 2002
J Appl Physiol, 10.1152/jap.01121.2001
Submitted on November 8, 2001
Accepted on May 8, 2002
1 Department of Respiratory Medicine, Hannover Medical School, Hannover, Germany
2 Drug Research and Clinical Inhalation, Fraunhofer Institute of Toxicology and Aerosol Research, Hannover, Germany
3 Department of Respiratory Medicine, Hannover Medical School, Hannover, Germany; Drug Research and Clinical Inhalation, Fraunhofer Institute of Toxicology and Aerosol Research, Hannover, Germany
4 Dept. of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, PA, USA
5 Department of Anatomy, Hannover Medical School, Hannover, Germany
* To whom correspondence should be addressed. E-mail: thomasglaab{at}web.de.
This study was designed to evaluate the value and applicability of tidal breathing pattern analysis to assess bronchoconstriction in conscious rats. Using noninvasive head-out body plethysmography and the decrease in tidal midexpiratory flow (EF50), we measured airway responsiveness (AR) to inhaled acetylcholine and allergen in conscious Brown-Norway rats, followed by invasive determination of pulmonary conductance (GL) and EF50 in anesthetized rats. Dose-response studies to acetylcholine showed that noninvasively recorded EF50 closely reflected the dose-dependent decreases observed with the invasive monitoring of simultaneously measured GL and EF50. After sensitization and intratracheal boost to ovalbumin or saline, rats were assessed for early and late airway responsiveness to aerosolized ovalbumin. Ovalbumin aerosol challenge resulted in early and late AR in allergen-sensitized rats, whereas controls were unresponsive. The allergen-specific AR, as measured noninvasively by EF50 was similar in degree compared with invasively recorded EF50 and GL, and was associated with enhanced IgE and airway inflammation. We conclude that EF50 is a noninvasive and physiologically valid index of bronchoconstriction in a rat model of asthma.
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