Journal of Applied Physiology AJP: Cell Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Appl Physiol (March 24, 2005). doi:10.1152/japplphysiol.01098.2004
This Article
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
99/2/528    most recent
01098.2004v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, L.
Right arrow Articles by Nyomba, B.L.G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, L.
Right arrow Articles by Nyomba, B.L.G.
Submitted on October 1, 2004
Accepted on March 22, 2005

In Vivo Insulin Signaling through PI 3-kinase is Impaired in Skeletal Muscle of Adult Rat Offspring Exposed to Ethanol in Utero

Li Chen1, Xing-Hai Yao2, and B.L.G. Nyomba3*

1 Department of Internal Medicine, University of Manitoba, Winnipeg, Manitoba, Canada; Department of Pharmacology, Jilin University, Changchun, Jilin, China
2 Department of Internal Medicine, University of Manitoba, Winnipeg, Manitoba, Canada
3 Department of Internal Medicine, University of Manitoba, Winnipeg, Manitoba, Canada; Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada

* To whom correspondence should be addressed. E-mail: bnyomba{at}cc.umanitoba.ca.

It is now known that prenatal ethanol (EtOH) exposure is associated with impaired glucose tolerance and insulin resistance in the rat offspring, but the underlying mechanism(s) is not known. To test the hypothesis that in vivo insulin signaling through PI 3-kinase is reduced in skeletal muscle of adult rat offspring exposed to EtOH in utero, we gave insulin intravenously to these rats and probed steps in the PI 3-kinase insulin signaling pathway. Following insulin treatment, EtOH-exposed rats had decreased tyrosine phosphorylation of the insulin receptor {beta}-subunit and of insulin receptor substrate-1 (IRS-1), as well as reduced IRS-1-associated PI 3-kinase in the gastrocnemius muscle, compared with control rats. There was no significant difference in basal or insulin-stimulated Akt activity between EtOH-exposed rats and controls. Insulinstimulated PKC{zeta} phosphorylation and membrane association were reduced in EtOHexposed rats compared with controls. Muscle insulin binding and peptide contents of insulin receptor, IRS-1, p85 subunit of PI 3-kinase, Akt/protein kinase B and atypical protein kinase C isoform {zeta} (PKC{zeta}) were not different between EtOH-exposed rats and controls. Thus, insulin resistance in rat offspring exposed to EtOH in utero may be explained, at least in part, by impaired insulin signaling through the PI-3 kinase pathway in skeletal muscle.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
X.-H. Yao and B. L. G. Nyomba
Hepatic insulin resistance induced by prenatal alcohol exposure is associated with reduced PTEN and TRB3 acetylation in adult rat offspring
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2008; 294(6): R1797 - R1806.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
X.-H. Yao and B. L. Gregoire Nyomba
Abnormal glucose homeostasis in adult female rat offspring after intrauterine ethanol exposure
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2007; 292(5): R1926 - R1933.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
K. Dembele, X.-H. Yao, L. Chen, and B. L. G. Nyomba
Intrauterine ethanol exposure results in hypothalamic oxidative stress and neuroendocrine alterations in adult rat offspring
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2006; 291(3): R796 - R802.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
X.-H. Yao, L. Chen, and B. L. G. Nyomba
Adult rats prenatally exposed to ethanol have increased gluconeogenesis and impaired insulin response of hepatic gluconeogenic genes
J Appl Physiol, February 1, 2006; 100(2): 642 - 648.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1948 by the American Physiological Society.