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J Appl Physiol (August 16, 2002). doi:10.1152/japplphysiol.01087.2001
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Articles in PresS, published online ahead of print August 16, 2002
J Appl Physiol, 10.1152/jap.01087.2001
Submitted on October 30, 2001
Accepted on July 30, 2002

Saccharomyces cerevisiae gene expression changes during rotating wall vessel suspension culture

Kelly Johanson1, Patricia L Allen2, Fawn C Lewis3, Luis A Cubano4, Linda E Hyman1, and Timothy G Hammond2*

1 Department of Biochemistry, Tulane University Health Sciences Center, New Orleans, LA, USA
2 Department of Medicine, Tulane University Health Sciences Center, New Orleans, LA, USA; Tulane/VA Environmental Astrobiology Center, Tulane University Health Sciences Center, New Orleans, LA, USA; VA Medical Center, New Orleans, LA, USA
3 Department of Surgery, Tulane University Health Sciences Center, New Orleans, LA, USA
4 Department of Medicine, Tulane University Health Sciences Center, New Orleans, LA, USA; Tulane/VA Environmental Astrobiology Center, Tulane University Health Sciences Center, New Orleans, LA, USA

* To whom correspondence should be addressed. E-mail: thammond{at}tulane.edu.

This study utilizes Saccharomyces cerevisiae to study genetic responses to suspension culture. The suspension culture system used in this study is the high aspect ratio vessel (HARV), one type of the rotating wall vessel (RWV), that provides a high rate of gas exchange necessary for rapidly diving cells. Cells were grown in the HARV, and DNA microarray and metabolic analyses were used to determine the resulting changes in yeast gene expression. A significant number of genes were found to be up or down regulated by at least two-fold as a result of rotational growth. Using Gibbs promoter alignment, clusters of genes were examined for promoter elements mediating these genetic changes. Candidate binding motifs similar to the Rap1p binding site and the Stress Responsive Element (STRE) were identified in the promoter regions of differentially regulated genes. This study shows that, as in higher order organisms, Saccharomyces cerevisiae changes gene expression in response to rotational culture and also provides clues for investigations into the signaling pathways involved in gravitational response.




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