Journal of Applied Physiology AJP: Endocrinology and Metabolism
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Appl Physiol (April 5, 2002). doi:10.1152/japplphysiol.01083.2001
This Article
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
93/1/311    most recent
01083.2001v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chicoine, L. G
Right arrow Articles by Walker, B. R
Right arrow Search for Related Content
PubMed
Right arrow Articles by Chicoine, L. G
Right arrow Articles by Walker, B. R

Articles in PresS, published online ahead of print April 5, 2002
J Appl Physiol, 10.1152/jap.01083.2001
Submitted on October 30, 2001
Accepted on March 27, 2002

Developmental Differences in Pulmonary eNOS Expression In Response to Chronic Hypoxia in the Rat

Louis G Chicoine1*, Jose W Avitia2, Cody Deen2, Leif D Nelin1, Scott Earley3, and Benjimen R Walker3

1 Department of Pediatrics, University of New Mexico, Albuquerque, New Mexico, USA; Departments of Cell Biology and Physiology, University of New Mexico, Albuquerque, New Mexico, USA
2 Department of Pediatrics, University of New Mexico, Albuquerque, New Mexico, USA
3 Departments of Cell Biology and Physiology, University of New Mexico, Albuquerque, New Mexico, USA

* To whom correspondence should be addressed. E-mail: lchicoine{at}salud.unm.edu.

Chronic hypoxia (CH) increases pulmonary endothelial nitric oxide synthase (eNOS) protein levels in adult rats, but decreases eNOS protein levels in neonatal pigs. We hypothesized that this differing response to CH is due to developmental rather than species differences. Adult and neonatal rats were placed in either hypobaric-hypoxia or normoxia for 14 days. At that time, body weight (BW), hematocrit (Hct), plasma nitrite/nitrate (NOx-), right ventricular (RV) and total ventricular (T) heart weights were measured. Percent pulmonary artery wall area (% wall area) of 20-50 µm and 51-100 µm arteries were also determined. Total lung protein extracts were assayed for eNOS levels using immune-blot analysis. Compared to their respective normoxic controls, both adult and neonatal hypoxic groups demonstrated significantly decreased BW, elevated Hct, and elevated RV/T ratios. Both, adult and neonatal hypoxic groups also demonstrated significantly larger % wall area compared to their respective normoxic controls. Hypoxic adult pulmonary eNOS protein and plasma NOx- were significantly greater than levels found in normoxic adults. In contrast, hypoxic neonatal pulmonary eNOS protein and plasma NOx- were significantly less compared to normoxic neonates. We conclude that there is a developmental difference in eNOS expression and NO production in response to CH.




This article has been cited by other articles:


Home page
 Reproductive SciencesHome page
T. R. Monau, V. E. Vargas, N. King, S. M. Yellon, D. A. Myers, and C. A. Ducsay
Long-Term Hypoxia Increases Endothelial Nitric Oxide Synthase Expression in the Ovine Fetal Adrenal
Reproductive Sciences, September 1, 2009; 16(9): 865 - 874.
[Abstract] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
L. G. Chicoine, M. L. Paffett, M. R. Girton, M. J. Metropoulus, M. S. Joshi, J. A. Bauer, L. D. Nelin, T. C. Resta, and B. R. Walker
Maturational changes in the regulation of pulmonary vascular tone by nitric oxide in neonatal rats
Am J Physiol Lung Cell Mol Physiol, November 1, 2007; 293(5): L1261 - L1270.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
C. D. Fike, Y. Zhang, and M. R. Kaplowitz
Thromboxane inhibition reduces an early stage of chronic hypoxia-induced pulmonary hypertension in piglets
J Appl Physiol, August 1, 2005; 99(2): 670 - 676.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. G. Chicoine, E. Tzeng, R. Bryan, S. Saenz, M. L. Paffett, J. Jones, C. R. Lyons, T. C. Resta, L. D. Nelin, and B. R. Walker
Intratracheal adenoviral-mediated delivery of iNOS decreases pulmonary vasoconstrictor responses in rats
J Appl Physiol, November 1, 2004; 97(5): 1814 - 1822.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
E. Villamor, C. G. A. Kessels, K. Ruijtenbeek, R. J. van Suylen, J. Belik, J. G. R. De Mey, and C. E. Blanco
Chronic in ovo hypoxia decreases pulmonary arterial contractile reactivity and induces biventricular cardiac enlargement in the chicken embryo
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2004; 287(3): R642 - R651.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
C. D. Fike, J. L. Aschner, Y. Zhang, and M. R. Kaplowitz
Impaired NO signaling in small pulmonary arteries of chronically hypoxic newborn piglets
Am J Physiol Lung Cell Mol Physiol, June 1, 2004; 286(6): L1244 - L1254.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Earley and B. R. Walker
Increased nitric oxide production following chronic hypoxia contributes to attenuated systemic vasoconstriction
Am J Physiol Heart Circ Physiol, May 1, 2003; 284(5): H1655 - H1661.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. E. Turley, L. D. Nelin, M. R. Kaplowitz, Y. Zhang, and C. D. Fike
Exhaled NO is reduced at an early stage of hypoxia-induced pulmonary hypertension in newborn piglets
Am J Physiol Lung Cell Mol Physiol, March 1, 2003; 284(3): L489 - L500.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1966 by the American Physiological Society.