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J Appl Physiol (January 18, 2002). doi:10.1152/japplphysiol.01069.2001
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Articles in PresS, published online ahead of print January 18, 2002
J Appl Physiol, 10.1152/jap.01069.2001
Submitted on October 24, 2001
Accepted on December 3, 2001

Changes in MCT1, MCT4 and LDH expression are tissue specific in rats after long-term hypobaric hypoxia

Grant B McClelland1 and George A Brooks1*

1 Integrative Biology, University of California, Berkeley, Berkeley, CA, USA

* To whom correspondence should be addressed. E-mail: gbrooks{at}socrates.berkeley.edu.

Little is known about the effect of chronic hypobaric hypoxia on the enzymes and transporters involved in lactate metabolism. We looked at the protein expression of monocarboxylate transporters MCT 1, MCT 2 and MCT 4, along with total lactate dehydrogenase (LDH) and LDH isozymes in skeletal muscle, cardiac muscle and liver. Expression of these components of the lactate shuttle affect the ability to transport and oxidize lactate. We hypothesized that the expression of MCTs and LDH would increase after acclimation to high altitude (HA). The response to acclimation to HA was, however, tissue specific. As well, the response was different in whole muscle (MU) and mitochondrial-enriched (MI) fractions. Heart, soleus and plantaris muscles showed the greatest response to HA. Acclimation resulted in a 34% increase in MCT 4 in heart and a decrease in MCT 1 (-47%) and MCT 4 (-47%) in plantaris MU. In MI fractions the heart had an increase (+40%) and soleus a decrease (-40%) in LDH. HA also had a significant effect on the LDH isozyme composition of both the MU and MI fractions. Mitochondrial density was decreased in both the soleus (-17%) and plantaris (-44%) as the result of chronic hypoxia. We conclude that chronic hypoxia had a tissue-specific effect on MCTs and LDH [that form the lactate shuttle] but did not produce a consistent increase in these components in all tissues.




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