Journal of Applied Physiology Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Appl Physiol (October 20, 2005). doi:10.1152/japplphysiol.01060.2005
This Article
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
100/4/1224    most recent
01060.2005v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wagner, E. M.
Right arrow Articles by McClintock, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wagner, E. M.
Right arrow Articles by McClintock, J.
Submitted on August 30, 2005
Accepted on October 18, 2005

Changes in lung permeability after chronic pulmonary artery obstruction

Elizabeth M. Wagner1*, Gulnura Karagulova1, John Jenkins1, John Bishai1, and Jessica McClintock1

1 Department of Medicine, Johns Hopkins University, Baltimore, MD, USA; Environmental Health Sciences, Johns Hopkins University, Baltimore, MD, USA

* To whom correspondence should be addressed. E-mail: wagnerem{at}jhmi.edu.

We have shown that left pulmonary artery ligation (LPAL) in mice causes a prompt angiogenic response, with new systemic vessels from intercostal arteries penetrating the pleura within 6 days. Since angiogenic vessels in other organs have been shown to exhibit increased permeability, we studied vascular permeability (Evans blue dye extravasation, lung wet wt/dry wt, and lavaged protein) in naive C57Bl/6 mice and 4hrs, 14 and 21 days after LPAL (4-6 mice/time point). We also measured radiolabel clearance as an index of functional perfusion after LPAL. Tracer clearance from the left lung was maximal by 6 days after LPAL and not different from right lungs. Thus, a functional vasculature is established before 6 days of LPAL that results in normal tracer clearance. By 21 days after LPAL, Evans blue-albumin was significantly increased in the left lung relative to both 4 hrs (no vasculature) and 14 days after LPAL. Only after 21 days of LPAL, was left lung wet wt/dry wt significantly different from naive lungs. Additionally, lavaged protein was significantly increased both 4 hrs and 21 days after LPAL relative to control mice. Thus, using 3 different methods, results consistently demonstrated increased permeability to protein and water 21 days after LPAL. Although changes in surface area of perfusion might affect the interpretation of these results, blood flow measured with labeled microspheres indicated no change in left lung perfusion between 14 and 21 days of LPAL. Thus, the lung vasculature, remodeled as a consequence of chronic pulmonary artery obstruction, demonstrates increased water and protein permeability.




This article has been cited by other articles:


Home page
 Exp. Biol. Med.Home page
W. Jin, H. Wang, Y. Ji, L. Zhu, W. Yan, L. Qiao, and H. Yin
Genetic Ablation of Nrf2 Enhances Susceptibility to Acute Lung Injury After Traumatic Brain Injury in Mice
Experimental Biology and Medicine, February 1, 2009; 234(2): 181 - 189.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Bhattacharya
Lung neovascularization: a tale of two circulations
Am J Physiol Lung Cell Mol Physiol, March 1, 2008; 294(3): L417 - L418.
[Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. Sanchez, A. Moldobaeva, J. McClintock, J. Jenkins, and E. Wagner
The role of CXCR2 in systemic neovascularization of the mouse lung
J Appl Physiol, August 1, 2007; 103(2): 594 - 599.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. J. Fields, J. M. Bishai, W. Mitzner, and E. M. Wagner
Effects of pulmonary ischemia on lung morphology
Am J Physiol Lung Cell Mol Physiol, July 1, 2007; 293(1): L254 - L258.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
P. Herve, E. Fadel, P. Herve, and E. Fadel
Systemic neovascularization of the lung after pulmonary artery occlusion: "decoding the Da Vinci code"
J Appl Physiol, April 1, 2006; 100(4): 1101 - 1102.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1948 by the American Physiological Society.