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1 Departement de physiologie des interactions (EA701), Institut de biologie, Bvd Henri IV, 34 060 Montpellier, France
2 700 av du pic saint loup, EA 2991 Efficience et deficience motrices, 34 090 Montpellier, France
* To whom correspondence should be addressed. E-mail: thomasclaire{at}wanadoo.fr.
The present study investigated whether muscular monocarboxylate transporters (MCT) 1 and 4 contents are related to the blood lactate removal after supramaximal exercise, fatigue indexes measured during different supramaximal exercises, and muscle oxidative parameters in fifteen humans with different training status. Lactate recovery curves were obtained after a 1-min all-out exercise. A bi-exponential time function was then used to determine the velocity constant of the slow phase (
2) which denoted the blood lactate removal ability. Fatigue indexes were calculated during 1-min all-out (FIAO) and repeated 10-s (FISprint) cycling sprints. Biopsies were taken from the vastus lateralis muscle. MCT1 and MCT4 contents were quantified by Western blots, and maximal muscle oxidative capacity (Vmax) was evaluated with pyruvate + malate and glutamate + malate as substrates. The results showed that the blood lactate removal ability (i.e. 2) after a 1-min all-out test was significantly related to MCT1 content (r = 0.70, P < 0.01), but not to MCT4 (r = 0.50, P > 0.05). However, greater MCT1 and MCT4 contents were negatively related with a reduction of blood lactate concentration at the end of 1-min all-out exercise (r = - 0.56, and r = - 0.61, P < 0.05, respectively). Among skeletal muscle oxidative indexes, we only found a relationship between MCT1 and glutamate + malate Vmax (r = 0.63, P < 0.05). Furthermore, MCT1 content, but not MCT4, was inversely related to FIAO (r = - 0.54, P < 0.05) and FISprint (r = - 0.58, P < 0.05). We concluded that skeletal muscle MCT1 expression was associated with the velocity constant of net blood lactate removal after a 1-min all-out test and with the fatigue indexes. It is proposed that MCT1 expression may be important for blood lactate removal after supramaximal exercise based to the existence of lactate shuttles and, in turn in favor of a better tolerance to muscle fatigue.
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