Journal of Applied Physiology Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Appl Physiol (August 20, 2004). doi:10.1152/japplphysiol.01006.2003
This Article
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
98/1/242    most recent
01006.2003v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ainslie, K. M
Right arrow Articles by Tarbell, J. M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ainslie, K. M
Right arrow Articles by Tarbell, J. M
Submitted on September 15, 2003
Accepted on August 2, 2004

Vascular smooth muscle cell glycocalyx influences shear stress-mediated contractile response

Kristy M Ainslie1*, Jeffrey S Garanich2, Randal O Dull3, and John M Tarbell4

1 Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA
2 Department of Chemical Engineering, The Pennsylvania State University, University Park, PA, USA; Deparment of Biomedical Engineering, The City College of New York / CUNY, New York, NY, USA
3 Department of Anesthesiology, The University of Utah, Salt Lake City, UT, USA
4 Deparment of Biomedical Engineering, The City College of New York / CUNY, New York, NY, USA

* To whom correspondence should be addressed. E-mail: kma169{at}psu.edu.

This study addressed the influence of the rate of shear stress application on aortic smooth muscle cell contraction and the role of specific glycosaminoglycans in this mechanotransduction. Rat aortic smooth muscle cells (SMCs) were exposed to either a step increase in shear stress (0 to 25 dynes/cm2) or a ramp increase in shear stress (0 to 25 dynes/cm2 over 5 minutes) in a parallel plate flow chamber, and cell contraction was characterized by cell area reduction. SMCs contracted at levels similar to those reported previously and equally in response to both a step and ramp increase in shear stress. When the cells were pre-treated with heparinase III or chondroitinase ABC to remove glycosaminoglycans (GAGs), heparan sulfate and chondroitin sulfate, respectively, from the glycocalyx, the contraction response to increases in shear stress was significantly inhibited. These studies indicate that specific components of the SMC glycocalyx play an important role in the mechanotransduction of shear stress into a contractile response and that the rate of application of shear stress does not affect the SMC contraction.




This article has been cited by other articles:


Home page
 Exp. Biol. Med.Home page
K. A. Addae-Mensah and J. P. Wikswo
Measurement Techniques for Cellular Biomechanics In Vitro
Experimental Biology and Medicine, July 1, 2008; 233(7): 792 - 809.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
Y. Yao, A. Rabodzey, and C. F. Dewey Jr.
Glycocalyx modulates the motility and proliferative response of vascular endothelium to fluid shear stress
Am J Physiol Heart Circ Physiol, August 1, 2007; 293(2): H1023 - H1030.
[Abstract] [Full Text] [PDF]

Home page
 Nephrol Dial TransplantHome page
A. L. Rops, C. W. Jacobs, P. C. Linssen, J. B. Boezeman, J. F. Lensen, T. J. Wijnhoven, L. P. van den Heuvel, T. H. van Kuppevelt, J. van der Vlag, and J. H. Berden
Heparan sulfate on activated glomerular endothelial cells and exogenous heparinoids influence the rolling and adhesion of leucocytes
Nephrol. Dial. Transplant., April 1, 2007; 22(4): 1070 - 1077.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
G. Kojda and R. Hambrecht
Molecular mechanisms of vascular adaptations to exercise. Physical activity as an effective antioxidant therapy?
Cardiovasc Res, August 1, 2005; 67(2): 187 - 197.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. P. Stegemann, H. Hong, and R. M. Nerem
Mechanical, biochemical, and extracellular matrix effects on vascular smooth muscle cell phenotype
J Appl Physiol, June 1, 2005; 98(6): 2321 - 2327.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1966 by the American Physiological Society.