| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print January 11, 2002
J Appl Physiol, 10.1152/jap.01002.2001
Submitted on October 1, 2001
Accepted on January 4, 2002
1 Thermal and Mountain Medicine Division, US Army Research Institute of Environmental Medicine, Natick, MA, USA; Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital / Harvard Medical School, Boston, MA, USA
2 Thermal and Mountain Medicine Division, US Army Research Institute of Environmental Medicine, Natick, MA, USA
3 Partners Gene Array Technology Center, Brigham and Women's Hospital / Harvard Medical School, Boston, MA, USA
4 Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital / Harvard Medical School, Boston, MA, USA
* To whom correspondence should be addressed. E-mail: larry.sonna{at}na.amedd.army.mil, LSONNA@NATICK-CCMAIL.ARMY.MIL.
We studied the effect of heat shock on gene expression by normal human cells. Peripheral blood mononuclear cells (PBMCs) were obtained from healthy adults. Paired samples from each subject were subjected to either 20 minutes of heat shock (43°C) or control (37°C) conditions, then returned to 37°C. RNA was isolated 160 minutes later and five representative samples were analyzed on Affymetrix gene chip arrays containing ~12,600 probes. A biologically meaningful effect was defined as a statistically significant, 2-fold or greater difference in expression of sequences that were detected in all 5 experiments under control (downregulated sequences) or heat shock (upregulated sequences) conditions. Changes occurred in 420 sequences (239 increased by heat shock, 181 decreased) representing 376 Unigene numbers, in every functional category previously implicated in the heat shock response. By RT-PCR, we confirmed the findings for one upregulated gene (Rad, a G-protein) and one downregulated gene (osteopontin, a cytokine). We conclude that heat shock causes extensive gene expression changes in PBMCs, affecting all functional categories of the heat shock response.
This article has been cited by other articles:
|
|
 |
|
  I. S. Singh, A. Gupta, A. Nagarsekar, Z. Cooper, C. Manka, L. Hester, I. J. Benjamin, J.-r. He, and J. D. Hasday Heat Shock Co-Activates Interleukin-8 Transcription Am. J. Respir. Cell Mol. Biol., August 1, 2008; 39(2): 235 - 242. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. P. McClung, J. D. Hasday, J.-r. He, S. J. Montain, S. N. Cheuvront, M. N. Sawka, and I. S. Singh Exercise-heat acclimation in humans alters baseline levels and ex vivo heat inducibility of HSP72 and HSP90 in peripheral blood mononuclear cells Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2008; 294(1): R185 - R191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Espinoza, J. A. Goodrich, and J. F. Kugel Characterization of the structure, function, and mechanism of B2 RNA, an ncRNA repressor of RNA polymerase II transcription RNA, April 1, 2007; 13(4): 583 - 596. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Selsby, S. Rother, S. Tsuda, O. Pracash, J. Quindry, and S. L. Dodd Intermittent hyperthermia enhances skeletal muscle regrowth and attenuates oxidative damage following reloading J Appl Physiol, April 1, 2007; 102(4): 1702 - 1707. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Sonna, M. M. Kuhlmeier, H. C. Carter, J. D. Hasday, C. M. Lilly, and K. D. Fairchild Effect of moderate hypothermia on gene expression by THP-1 cells: a DNA microarray study Physiol Genomics, September 14, 2006; 26(1): 91 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Moran, L. Eli-Berchoer, Y. Heled, L. Mendel, M. Schocina, and M. Horowitz Heat intolerance: does gene transcription contribute? J Appl Physiol, April 1, 2006; 100(4): 1370 - 1376. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ilany, P. J. Bilan, S. Kapur, J. S. Caldwell, M.-E. Patti, A. Marette, and C. R. Kahn Overexpression of Rad in muscle worsens diet-induced insulin resistance and glucose intolerance and lowers plasma triglyceride level PNAS, March 21, 2006; 103(12): 4481 - 4486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. T. Selsby and S. L. Dodd Heat treatment reduces oxidative stress and protects muscle mass during immobilization Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2005; 289(1): R134 - R139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Lilly, H. Tateno, T. Oguma, E. Israel, and L. A. Sonna Effects of Allergen Challenge on Airway Epithelial Cell Gene Expression Am. J. Respir. Crit. Care Med., March 15, 2005; 171(6): 579 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Horowitz, L. Eli-Berchoer, I. Wapinski, N. Friedman, and E. Kodesh Stress-related genomic responses during the course of heat acclimation and its association with ischemic-reperfusion cross-tolerance J Appl Physiol, October 1, 2004; 97(4): 1496 - 1507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Sonna, C. B. Wenger, S. Flinn, H. K. Sheldon, M. N. Sawka, and C. M. Lilly Exertional heat injury and gene expression changes: a DNA microarray analysis study J Appl Physiol, May 1, 2004; 96(5): 1943 - 1953. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Wood, L. Tchack, G. Angelo, R. E. Pratt, and L. A. Sonna DNA microarray analysis of vitamin D-induced gene expression in a human colon carcinoma cell line Physiol Genomics, April 13, 2004; 17(2): 122 - 129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Lampe, S. B. Stepaniants, M. Mao, J. P. Radich, H. Dai, P. S. Linsley, S. H. Friend, and J. D. Potter Signatures of Environmental Exposures Using Peripheral Leukocyte Gene Expression: Tobacco Smoke Cancer Epidemiol. Biomarkers Prev., March 1, 2004; 13(3): 445 - 453. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
