Journal of Applied Physiology AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

J Appl Physiol (October 6, 2005). doi:10.1152/japplphysiol.00975.2005
This Article
Right arrow Full Text (PDF) Free
Right arrow All Versions of this Article:
100/2/631    most recent
00975.2005v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fang, Q.
Right arrow Articles by Mayhan, W. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fang, Q.
Right arrow Articles by Mayhan, W. G.
Submitted on August 10, 2005
Accepted on October 4, 2005

Inhibition of NAD(P)H Oxidase Improves Impaired Reactivity of Pial Arterioles During Chronic Exposure to Nicotine

Qin Fang1, Hong Sun1, Denise M. Arrick1, and William G. Mayhan1*

1 Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, USA

* To whom correspondence should be addressed. E-mail: wgmayhan{at}unmc.edu.

Our goals were to determine whether chronic exposure to nicotine alters nitric oxide synthase (NOS)-dependent reactivity of cerebral (pial) arterioles and to identify a potential role for NAD(P)H oxidase in impaired NOS-dependent responses during chronic exposure to nicotine. We measured in vivo diameter of pial arterioles to NOS-dependent (acetylcholine and 5'-adenosine diphosphate (ADP)) and -independent (nitroglycerin) agonists in saline treated rats and rats chronically treated with nicotine (2mg/kg/day for 2 weeks via an osmotic minipump). We found that NOS-dependent, but not -independent, vasodilatation was impaired in nicotine treated compared to saline treated rats. In addition, the production of superoxide anion (lucigenin chemiluminescence) was increased in rats treated with nicotine when compared to saline treated rats. Further, using Western blot analysis, we found that chronic exposure to nicotine increased p47phox protein in the parietal cortex. Finally, we found that apocynin (40 mg/kg/day in the drinking water to inhibit NAD(P)H oxidase alleviated impaired NOSdependent cerebral vasodilatation in nicotine treated rats, did not alter NOS-dependent responses in saline treated rats, and did not alter NOS-independent reactivity in saline or nicotine treated rats. These findings suggest that chronic exposure to nicotine impairs NOS-dependent dilatation of pial arterioles by a mechanism that appears to be related to the formation of superoxide anion via activation of NAD(P)H oxidase.




This article has been cited by other articles:


Home page
 Nicotine Tob ResHome page
W. G. Mayhan, D. M. Arrick, G. M. Sharpe, and H. Sun
Nitric oxide synthase-dependent responses of the basilar artery during acute infusion of nicotine
Nicotine Tob Res, March 1, 2009; 11(3): 270 - 277.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. H. Fabian, J. R. Perez-Polo, and T. A. Kent
Perivascular nitric oxide and superoxide in neonatal cerebral hypoxia-ischemia
Am J Physiol Heart Circ Physiol, October 1, 2008; 295(4): H1809 - H1814.
[Abstract] [Full Text] [PDF]

Home page
 Journal of Renin-Angiotensin-Aldosterone SystemHome page
H. Iida, M. Iida, M. Takenaka, N. Fukuoka, and S. Dohi
Rho-kinase inhibitor and Nicotinamide Adenine Dinucleotide PHosphate oxidase inhibitor prevent impairment of endothelium-dependent cerebral vasodilation by acute cigarette smoking in rats
Journal of Renin-Angiotensin-Aldosterone System, June 1, 2008; 9(2): 89 - 94.
[Abstract] [PDF]

Home page
 J. Appl. Physiol.Home page
D. M. Arrick and W. G. Mayhan
Acute infusion of nicotine impairs nNOS-dependent reactivity of cerebral arterioles via an increase in oxidative stress
J Appl Physiol, December 1, 2007; 103(6): 2062 - 2067.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1948 by the American Physiological Society.