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Articles in PresS, published online ahead of print January 11, 2002
J Appl Physiol, 10.1152/jap.00950.2001
Submitted on September 14, 2001
Accepted on January 10, 2002
1 Department of Kinesiology, Texas Woman's University, Denton, Texas, USA
2 Center for Exercise Science, University of Florida, Gainesville, Florida, USA; Department of Kinesiology, Texas Woman's University, Denton, Texas, USA
* To whom correspondence should be addressed. E-mail: dcriswell{at}hhp.ufl.edu.
The purpose of this study was to determine the necessity of nitric oxide (NO) for hypertrophy and fiber type transition in overloaded (OL) skeletal muscle. Endogenous NO production was blocked by administering N-nitro-L-arginine methyl ester (L-NAME; 0.75 mg/ml; ~100mg/kg/day) in drinking water. Thirty-eight female Sprague-Dawley rats (~250g) were randomly divided into four groups: Control-NonOL, Control-OL, L-NAME-NonOL, and L-NAME-OL. Chronic overload of the plantaris was induced bilaterally by surgical removal of the gastrocnemius and soleus. Rats in the NonOL groups received sham surgeries. L-NAME treatment began 24 hrs before surgery and continued until the rats were killed, 14 days post-surgery. Although OL induced hypertrophy in both Control (+76%) and L-NAME (+39%) conditions (P<0.05), mean plantaris/body mass ratio in the L-NAME-OL group was significantly lower (P<0.05) than the Control-OL group. Microphotometric analysis of histochemically determined fiber types revealed increases in cross-sectional area (P<0.05) for all fiber types (type I, IIA, and IIB/X) in the OL plantaris from Control rats, while L-NAME-OL rats exhibited increases only in type I and IIB/X fibers. SDS-PAGE analysis of myosin heavy chain (MHC) composition in the plantaris indicated a significant (P<0.05) OL-effect in the Control rats. Specifically, the mean proportion of type I MHC increased 6% (P<0.05) while the proportion of type IIb MHC decreased ~9% (P<0.05). No significant OL effects on MHC profile were observed in the L-NAME rats. These data support a role of NO in overload-induced skeletal muscle hypertrophy and fiber type transition.
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