This study examined if the elasticity of the tendinous tissues plays an important role in human locomotion by improving the power output and efficiency of skeletal muscle. Ten subjects performed one-leg drop jumps (DJ) from different dropping heights with a constant rebound height. The fascicle length of the vastus lateralis muscle (VL) was measured by using real-time ultrasonography during DJ. In the braking phase of the DJ fascicle lengthening decreased and the tendinous tissue lengthening increased with increased dropping intensity. In the subsequent push-off phase, the shortening of tendinous tissues increased with higher dropping intensity. The averaged electromyographic activities of the preactivation and braking phases increased and those of the push-off phase decreased as the drop height were increased. With higher dropping height but constant submaximal rebound jump, the stretched tendinous tissue length increased with less stretched fascicle during the braking phase. In the subsequent push-off phase, the recoil of tendinous tissues became greater. These results suggest that the increased prestretch intensity has considerable influence on the process of storage and subsequent recoil of the elastic energy during the stretch-shortening cycle action.