Journal of Applied Physiology
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J Appl Physiol (January 18, 2002). doi:10.1152/japplphysiol.00926.2001
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Articles in PresS, published online ahead of print January 18, 2002
J Appl Physiol, 10.1152/jap.00926.2001
Submitted on September 5, 2001
Accepted on December 15, 2001

Effect of exercise on T-lymphocyte function: A comparison of proliferation in PBMC and natural killer cell depleted PBMC culture

Katherine J Green1*, David G Rowbottom1, and Laurel T Mackinnon2

1 Department of Human Movement Studies, Queensland University of Technology, Brisbane, Queensland, Australia
2 Department of Human Movement Studies, University of Queensland, Brisbane, Queensland, Australia

* To whom correspondence should be addressed. E-mail: k.mclean{at}qut.edu.au.

This study utilised recently developed MicroBead technology to remove natural killer (NK) cells from peripheral blood mononuclear cells (PBMC) preparations to determine the effect of acute exercise on T-lymphocyte function independent of changes in lymphocyte subpopulations. Twelve well-trained male runners completed a 60 min exercise trial at 95% ventilatory threshold and a no-exercise control trial. Six blood samples were taken at each session; before exercise, mid exercise, immediately after and 30 min, 60 min and 90 min after exercise. Isolated PBMC and NK cell depleted PBMC were stimulated with the mitogen PHA. Cellular proliferation was assessed using 3-(4,5-dimethlthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye uptake. In the PBMC cultures there was a significantly lower mitogen response to PHA in exercise compared to the control condition immediately post exercise. There were no significant differences between the control and exercise conditions in NK cell depleted PBMC cultures or in the responses adjusted for the percentage of CD3 cells. The present findings do not support the view that T-lymphocyte function is reduced following exercise.




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