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1 August Krogh Institute, Copenhagen Muscle Research Center, Copenhagen, Denmark; Rigshospitalet, Copenhagen Muscle Research Center, Copenhagen, Denmark
2 Rigshospitalet, Copenhagen Muscle Research Center, Copenhagen, Denmark
* To whom correspondence should be addressed. E-mail: hpilegaard{at}aki.ku.dk.
Increasing evidence suggests that the myogenic regulatory factors (MRFs) and insulin-like growth factor (IGF)-I have important roles in the hypertrophy response observed after mechanical loading. We therefore hypothesized that a bout of heavy resistance training would affect the MRF and IGF-I mRNA levels in human skeletal muscle. Six male subjects completed four sets of 6-12 repetitions on a leg press and knee extensor machine separated by three minutes. Myogenin, MRF4, MyoD, IGF-IEac (isoforms a and c) and IGF-IEc (isoform c) mRNA levels were determined in the vastus lateralis muscle by RT-PCR before exercise, immediately after and 1, 2, 6, 24 and 48 h post-exercise. Myogenin, MyoD and MRF4 mRNA levels were elevated (P<0.005) by 100-400% 0-24 h post-exercise. IGF-IEac mRNA content decreased (P<0.005) by approximately 44% after 1 and 6 h of recovery. The IGF-IEc mRNA level was unaffected. The present study shows that Myogenin, MyoD and MRF4 mRNA levels are transiently elevated in human skeletal muscle after a single bout of heavy resistance training, supporting the idea that the MRFs may be involved in regulating hypertrophy and/or fiber type transitions. The results also suggest that IGF-IEa expression may be downregulated at the mRNA level during the initial part of recovery from resistance exercise.
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