| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Departments of Physiology & Biophysics, Mayo Clinic College of Medicine, Rochester, MN, USA
2 Department of Anesthesiology, Mayo Clinic College of Medicine, Rochester, MN, USA
3 Departments of Physiology & Biophysics, Mayo Clinic College of Medicine, Rochester, MN, USA; Department of Anesthesiology, Mayo Clinic College of Medicine, Rochester, MN, USA
* To whom correspondence should be addressed. E-mail: sieck.gary{at}mayo.edu.
Unilateral denervation (DNV) of the rat diaphragm muscle (DIAm) markedly alters expression of myosin heavy chain (MHC) isoforms. Following 2 weeks of DIAm DNV, MHC content per half-sarcomere decreases in fibers expressing MHC2X and MHC2B. We hypothesized that changes in MHC protein expression parallel changes in MHC mRNA expression. Relative MHC isoform mRNA levels were determined by Northern analysis following 1, 3, 7, and 14 days of DNV of the rat DIAm. MHC protein expression was determined by SDS-PAGE. Changes in MHC isoform protein and mRNA expression were not concurrent. Expression of MHCSlow and MHC2X mRNA isoforms decreased dramatically by 3d DNV, whereas that of MHC2A and MHC2B did not change. Expression of all MHC protein isoforms decreased by 3d DNV. We observed a differential effect of rat DIAm DNV on MHC isoform protein and mRNA expression. The time course of the changes in MHC isoform mRNA and protein expression suggests a predominant effect of altered protein turnover rates on MHC protein expression instead of altered transcription following DNV.
This article has been cited by other articles:
|
|
 |
|
  H. M. Argadine, N. J. Hellyer, C. B. Mantilla, W.-Z. Zhan, and G. C. Sieck The effect of denervation on protein synthesis and degradation in adult rat diaphragm muscle J Appl Physiol, August 1, 2009; 107(2): 438 - 444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. B. Mantilla and G. C. Sieck Key aspects of phrenic motoneuron and diaphragm muscle development during the perinatal period J Appl Physiol, June 1, 2008; 104(6): 1818 - 1827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Sieck, W.-Z. Zhan, Y.-S. Han, and Y. S. Prakash Effect of denervation on ATP consumption rate of diaphragm muscle fibers J Appl Physiol, September 1, 2007; 103(3): 858 - 866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Powers, A. N. Kavazis, and J. M. McClung Oxidative stress and disuse muscle atrophy J Appl Physiol, June 1, 2007; 102(6): 2389 - 2397. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. McClung, A. N. Kavazis, K. C. DeRuisseau, D. J. Falk, M. A. Deering, Y. Lee, T. Sugiura, and S. K. Powers Caspase-3 Regulation of Diaphragm Myonuclear Domain during Mechanical Ventilation-induced Atrophy Am. J. Respir. Crit. Care Med., January 15, 2007; 175(2): 150 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Geiger, J. P. Bailey, C. B. Mantilla, W.-Z. Zhan, and G. C. Sieck Mechanisms underlying myosin heavy chain expression during development of the rat diaphragm muscle J Appl Physiol, December 1, 2006; 101(6): 1546 - 1555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Adreani, Z. B. Li, M. Lehar, L. L. Southwood, P. L. Habecker, P. W. Flint, and E. J. Parente Myosin heavy chain composition in normal and atrophic equine laryngeal muscle. Vet. Pathol., November 1, 2006; 43(6): 881 - 889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Aravamudan, C. B. Mantilla, W.-Z. Zhan, and G. C. Sieck Denervation effects on myonuclear domain size of rat diaphragm fibers J Appl Physiol, May 1, 2006; 100(5): 1617 - 1622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. DeRuisseau, R. A. Shanely, N. Akunuri, M. T. Hamilton, D. Van Gammeren, A. M. Zergeroglu, M. McKenzie, and S. K. Powers Diaphragm Unloading via Controlled Mechanical Ventilation Alters the Gene Expression Profile Am. J. Respir. Crit. Care Med., November 15, 2005; 172(10): 1267 - 1275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Siu and S. E. Alway Mitochondria-associated apoptotic signalling in denervated rat skeletal muscle J. Physiol., May 15, 2005; 565(1): 309 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E.-K. Pae, J. Wu, D. Nguyen, R. Monti, and R. M. Harper Geniohyoid muscle properties and myosin heavy chain composition are altered after short-term intermittent hypoxic exposure J Appl Physiol, March 1, 2005; 98(3): 889 - 894. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
