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Articles in PresS, published online ahead of print November 27, 2002
J Appl Physiol, 10.1152/jap.00833.2002
Submitted on September 13, 2002
Accepted on November 18, 2002
1 School of Exercise and Sport Science, The University of Sydney, Lidcombe, NSW, Australia
2 School of Molecular and Microbial Bioscience, University of Sydney, Sydney, NSW, Australia
3 Rayscan Imaging, Liverpool, NSW, Australia
* To whom correspondence should be addressed. E-mail: njohnson{at}ihug.com.au.
Vastus lateralis intramyocellular lipid (IMCL) content was assessed by 1H-magnetic resonance spectroscopy (1H-MRS) before and after prolonged time-trial cycling bouts of ~3 h duration. Six highly trained male cyclists completed a double-blind, randomized crossover design of two experimental trials following a strenuous exercise bout and 48 h of high (HC) (9.32 ± 0.08 g.kg-1.day-1) and low (LC) (0.59 ± 0.21 g.kg-1.day-1) dietary CHO. Resting IMCL content was significantly higher following LC versus HC (p < 0.01) and was reduced during exercise by 64% and 57%, respectively. IMCL was not different between conditions after exercise (p > 0.05). The ~2-fold increase in IMCL degradation in LC compared with HC suggests that higher rates of whole-body lipid metabolism in LC were in part attributable to a greater IMCL utilization. Four subjects experienced reductions of IMCL in excess of 70% during exercise. To our knowledge, this is the first study to report near depletion of intramyocellular lipid during prolonged cycling, indicating that IMCL, presumably the triacylglycerol component, may be exhausted by prolonged strenuous exercise.
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