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J Appl Physiol (December 21, 2006). doi:10.1152/japplphysiol.00821.2006
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Submitted on July 26, 2006
Accepted on December 16, 2006

Increased insulin-stimulated Akt pSer473 and cytosolic SHP2 protein abundance in human skeletal muscle following acute exercise and short-term training

Glenn D Wadley1*, Nicky Konstantopoulos2, Lance Macaulay3, Kirsten Howlett4, Andrew Garnham4, Mark Hargreaves5, and David Cameron-Smith4

1 School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria, Australia; Department of Physiology, The University of Melbourne, Melbourne, Victoria, Australia
2 Molecular and Health Technologies, CSIRO, Parkville, Victoria, Australia; Metabolic Research Unit, Deakin University, Geelong, Victoria, Australia
3 Molecular and Health Technologies, CSIRO, Parkville, Victoria, Australia
4 School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria, Australia
5 Department of Physiology, The University of Melbourne, Melbourne, Victoria, Australia

* To whom correspondence should be addressed. E-mail: gdwadley{at}unimelb.edu.au.

The purpose of the present study was to determine in human skeletal muscle if a single exercise bout and 7 days of consecutive endurance (cycling) training 1) increased insulin-stimulated Akt pSer473, and 2) altered the abundance of the protein tyrosine phosphatases (PTPases), PTP1B and SHP2. In healthy untrained males (n=8, 24±1yrs), glucose infusion rate (GIR) during a hyperinsulinemic euglycemic clamp, when compared with untrained values, was not improved 24 h following a single 60 min bout of endurance cycling, but was significantly increased (~ 30%, p<0.05) 24 h following completion of 7 days of exercise training. Insulin-stimulated Akt pSer473 was ~ 50% higher (p<0.05) 24 h following the acute bout of exercise, with this effect remaining after 7 days of training (p<0.05). Insulin-stimulated IR and IRS-1 tyrosine phosphorylation were not altered 24 h after acute exercise and short-term training. Insulin did not acutely regulate the localization of the PTPases, PTP1B or SHP2, although cytosolic protein abundance of SHP2 was increased (p<0.05; main effect) 24 h following acute exercise and short-term training. In conclusion, insulin-sensitive Akt pSer473 and cytosolic SHP2 protein abundance are higher after acute exercise and short-term training and this effect appears largely due to the residual effects of the last bout of prior exercise. The significance of exercise-induced alterations in cytosolic SHP2 and insulin-stimulated Akt pSer473 on the improvement in insulin sensitivity requires further elucidation.




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