Journal of Applied Physiology AJP: Endocrinology and Metabolism
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J Appl Physiol (March 14, 2003). doi:10.1152/japplphysiol.00783.2001
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Submitted on July 25, 2001
Accepted on March 1, 2003

Kinetic analysis of pulmonary neutrophil retention in vivo using the multiple indicator-dilution technique

Andreas J Schwab1, Agnes Salamand1, Yahye Merhi1, Andre Simard1, and Jocelyn Dupuis2*

1 Montreal General Hospital, McGill University Medical Clinic, Montreal, Quebec, Canada
2 Department of Medicine, Montreal Heart Institute & University of Montreal, Montreal, Quebec, Canada

* To whom correspondence should be addressed. E-mail: dupuisj{at}icm.umontreal.ca.

Multiple indicator-dilution experiments were performed in the lungs of 13 anesthetized dogs by simultaneous bolus injection of 111In-labeled neutrophils, 51Cr red blood cells (RBCs) and Evans-blue-labeled albumin. Concomitant counts of unlabeled neutrophils were similar in pulmonary artery and aortic blood samples, demonstrating a dynamic balance across the lungs in the physiological state. Outflow profiles of labeled neutrophils were analyzed based on a recirculatory pharmacokinetic model of labeled albumin. The outflow profiles of the recovered neutrophils were composed of a throughput component of circulating neutrophils and a component of reversibly marginated neutrophils. They were interpreted by a model incorporating neutrophil margination (transfer coefficient 0.195 ± 0.081 s-1), rapid demargination (0.054 ± 0.027 s-1) and transfer into a slow marginated pool (0.023 ± 0.018 s-1). It will be interesting to apply the analysis in future studies aimed at determining whether it could be a useful research tool to investigate the interactions between the pulmonary endothelium and neutrophils in physiological and diseased states.




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